中国药理学通报2012，Vol.28Issue(3)：342-346,5.DOI:10.3969/j.issn.1001-1978.2012.03.011
豚鼠高脂血症模型LDL-C代谢紊乱的机制研究
Mechanism of hyperlipidemic LDL-C metabolic disorder in the guinea pig
摘要
Abstract
Aim To establish the hyperlipidemic guinea pig model and investigate the mechanism of development. Methods Experimental hyperlipidemic guinea pig model induced by high-cholesterol diets was established. Serum lipids were determined after modeling 1, 2 and 4 weeks, and the concentration of ox-LDL was also determined by ELISA. Real-time polymerase chain reaction to measure the expressions of CYP7A1, FXR, LXRa and HMG-CoA reductase Mrna. LDL-R was measured by western blot. Results In comparison with control group, serum TC and LDL-C were significantly increased after fed with high cholesterol diet for 1 week, and as time went by, the serum lipids remained at a high level. The concentrations of serum ox-LDL and sd-LDL were also increased. The model group had a greater accumulation of total cholesterol inthe liver compared with the control group. The expression of CYP7AlmRNA had no change. The expression of HMG-CoA reductase Mrna occurred up-regulation. The Mrna levels of both hepatic farnesoid X receptor ( FXR ) and liver X receptor( LXR ) were increased in the high-cholesterol group. Surprisingly, these receptors were activated, but the cholesterol did not affect CYP7A1 transcript levels. The protein expression of LDL-R in model group was obviously higher than the control group. Conclusions The development of guinea pig hyperlipidemic model induced by high fat diets is related to the elimination disorder of the exogenous high cholesterol and LDL-C.关键词
豚鼠/高胆固醇血症/ox-LDL/sd-LDL/CYP7A1/FXR/LXRα/LDL-RKey words
guinea pig/ hyperlipidemia/ ox-LDL/ sd-LDL / CYP7Al/ FXR/ LXRa/ LDL-R分类
医药卫生引用本文复制引用
宋鑫,高南南,杨润梅,刘芳..豚鼠高脂血症模型LDL-C代谢紊乱的机制研究[J].中国药理学通报,2012,28(3):342-346,5.基金项目
北京市自然科学基金面上项目(No 7122112) （No 7122112）
