中国药理学通报2012，Vol.28Issue(3)：347-351,5.DOI:10.3969/j.issn.1001-1978.2012.03.012
应用胚胎干细胞试验模型对碱性成纤维细胞生长因子发育毒性的初步评价
Embryonic stem cell test for the study of the developmentaltoxicity of basic fibroblast growth factor
摘要
Abstract
Aim To research the developmental toxicity of basic Fibroblast Growth Factor ( bFGF ) by theembryonic stem cell test. Methods Mouse embryonic stem cells were cultured and their undifferentiationswere identified by the methods of morphology, alkaline phosphatase staining and RT-PCR. The cytotoxic effect of bFGF was detected by MTT assay. The effect of bF-GF on Sox-2 gene expression was determined by semi-quantitative RT-PCR. Result The IC50 of bFGF on3T3 was 24. 2 mg · L-1, while IC50 and ID50 on ESCwere 15. 2 mg · L -1 and 1. 7 mg · L -1 respectively. Conclusion The developmental toxicity of bFGF is e-valuated as weak embryotoxicity.关键词
小鼠胚胎干细胞/胚胎干细胞试验模型/碱性成纤维细胞生长因子/Sox-2胚胎毒性/发育毒性Key words
mouse embryonic stem cells/ embryonic stem cell test/ basic Fibroblast Growth Factor/ Sox-2/ embryonic toxicity/ developmental toxicity分类
医药卫生引用本文复制引用
许华,邓淑琴,何清,苏本金,江海香,黄亚东..应用胚胎干细胞试验模型对碱性成纤维细胞生长因子发育毒性的初步评价[J].中国药理学通报,2012,28(3):347-351,5.基金项目
国家自然科学基金资助项目(No 30973602) （No 30973602）
广东省科技计划项目(No 2011B031700009) （No 2011B031700009）
广州市科技计划项目(No 2010Y1-C851) （No 2010Y1-C851）
