中国药理学通报2012，Vol.28Issue(3)：366-371,6.DOI:10.3969/j.issn.1001-1978.2012.03.016
成纤维细胞生长因子-21改善胰岛素抵抗肝细胞对葡萄糖的吸收及机制
The effect of FGF-21 on the glucose uptakes andinsulin resistance in cultured human liver cells
摘要
Abstract
Aim To establish an insulin resistance cell model and evaluate the effects of FGF-21 on insulin resistance. Methods Human liver cells were administrated by high concentration of Dexamethasone and insulin. Glucose uptake activity of FGF-21 was examined by glucose oxidase and peroxidase( GOD-POD ) assay. The expressions of GLUT1 and the ERK1/2 signaling pathway were examined by Western blot. Results FGF-21 could stimulate glucose uptake of HL-7702 cells in a dose-dependent manner, suggesting that theglucose uptake assay was valid. Glucose uptake of HL-7702 cells stimulated by the mixture of FGF-21 and insulin was higher than that by FGF-21 or insulin solely. The FGF-21 protein was subsequently tested in IR cell models, showing that it could also stimulate glucose uptake of HL-7702 cells. The expressions of GLUT1 and the phosphorylation of ERK1/2 increased with the administration of FGF-21. The phosphorylation of ERK1/ 2 stimulated by FGF-21 was inhibited when PD98059, the specific inhibitor of ERK1/2, was added into thecell supernatant. In the IR model, FGF-21 could still increase the content of p-ERKl/2. Conclusions FGF-21 can stimulate glucose uptake of HL-7702 cells in a dose-dependent manner, and stimulate the glucose uptake of IR cell models. FGF-21 increases the ex-pression of GLUT1 and the phosphorylation of ERK1/2.关键词
FGF-21/肝细胞/地塞米松/胰岛素抵抗/GLUT1/ERK1/2信号Key words
FGF-21/ human liver cells/ dexametha- sone/ insulin resistance/ GLUT1/ ERK1/2 signaling pathway分类
医药卫生引用本文复制引用
李婷婷,高丽昌,唐禄,张健,李富勇,李海燕,王晓杰,李校堃..成纤维细胞生长因子-21改善胰岛素抵抗肝细胞对葡萄糖的吸收及机制[J].中国药理学通报,2012,28(3):366-371,6.基金项目
国家科技重大专项"重大新药创制"(No 2011ZX09102-004-03) （No 2011ZX09102-004-03）
