中国药理学通报2012，Vol.28Issue(3)：383-387,5.DOI:10.3969/j.issn.1001-1978.2012.03.020
SKF97541抑制大鼠骶髓后联合核神经元
SKF97541 inhibits spinal sacral dorsal commissural nucleus neurons of the rat
摘要
Abstract
Aim To investigate the possible actions of SKF97541, a selective GABAB receptor agonist, on sacral dorsal commissural nucleus ( SDCN ) neurons of spinal cord. Methods Conventional whole-cell recordings were conducted on SDCN neurons of acute spinal cord slice. Actions of SKF97541 on membrane potentials and action potential firing were investigated under current-clamp mode, glutamatergic synaptic transmission was checked under voltage-clamp mode. Results SDCN neurons membrane potentials were hyperpolar-ized and action potential firings were decreased by SKF97541( 0. 5 μmol · L-1 ) treatment. SKF97541 reduced frequency but not amplitude of glutamate-mediated miniature excitatory postsynaptic currents ( Mep-SCs ), indicating a presynaptic inhibition. Presynapticstimulation induced evoked postsynaptic potential ( Ep-SPs ). The amplitude of ePSPs was reduced by SKF97541, with an increase of paired-pulse ratio ( PPR ). All the above SKF97541 actions were largely abolished by a selective GABAB receptor antagonist, CGP52432, indicating SKF97541 action via GABAB receptors. Conclusions SKF97541 directly inhibits SDCN neurons by suppressing postsynaptic neuronal excitability and action potential firing. On the other hand, SKF97541 presynaptically inhibits glutamate release. Thus SKF97541 may modulate pain signal transmission and modulation at SDCN.关键词
脊髓/骶髓后联合核/谷氨酸/GABAB受体/SKF97541/全细胞记录/大鼠Key words
spinal cord/ sacral dorsal commissural nucleus/ glutamate/ GABAB receptors/ SKF97541/ whole-cell recordings/ rat分类
医药卫生引用本文复制引用
马红雨,宋世平,董磊,罗丹,吕建晓,杨鲲..SKF97541抑制大鼠骶髓后联合核神经元[J].中国药理学通报,2012,28(3):383-387,5.基金项目
国家自然科学基金资助课题(No 30000052) （No 30000052）
