中国药理学通报2012，Vol.28Issue(3)：388-392,5.DOI:10.3969/j.issn.1001-1978.2012.03.021
人参皂苷Rg1抗血管内膜增生与其抗氧化和上调eNOS表达作用的关系
Inhibitory effect of ginsenoside Rg1 on vascular neointimal hyperplasiainduced by balloon-injury in rats and the relation to its anti-oxidantaction and up-regulating eNOS expression
摘要
Abstract
Aim To investigate the mechanisms of theinhibitory effect of ginsenoside Rgl ( Rgl ) on vascularneointimal hyperplasia induced by balloon-injury in rats. Methods The balloon-injured rat carotid artery model was established by rubbing the endothelia with a balloon in male Sprague-Dawley rats. Animals were then injected with distilled water ( model group and sham operation group ) or Rgl 4 mg · kg'-1,8 mg ? Kg-1 and 16 mg · kg-1 for 14 consecutive days, then the injuried artery was taken for HE staining. The vascular intimal hyperplasia level was evidenced by his-topathological observation and the neointimal area as well as the ratio of neointimal area/media area was detected. For probing the mechanisms, the plasma super-oxide dismutase ( SOD ) activities and maleic dialde-hyde ( MDA ) levels were detected, and the expression of endothelial nitric oxide synthase ( eNOS ) mRNA levels were analyzed by Real-Time PCR. ResultsCompared with the endothelia rubbing model group, Rgl medication significantly improved the histopatho-logical changes induced by balloon-injury, decreased the elevated neointimal area and the ratio of neointimal area/ media area; Rgl administration could also increase the lowered plasma SOD activities, and decrease the elevated MDA l, Rgl could significantly up-regulate the eNOS mevels. When the doses were at 8 mg · kg-1 and 16 mg · kg'-1 RNA expression which was inhibited by balloon-injury. Conclusion The mechanisms by which Rgl inhibits the vascular neointimal hyperplasia induced by ballon-injury in rats may be related to its anti-oxidant actions and up-regulating eNOS expression.关键词
人参皂苷Rg1/球囊损伤/血管内膜增生/超氧化物歧化酶/丙二醛/内皮型一氧化氮合酶Key words
Ginsenoside Rgl/ balloon-injured/ vascular neointimal hyperplasia/ SOD/ MDA/ eNOS分类
医药卫生引用本文复制引用
高杨,吴芹,杨丹莉,邓江,黄燮南..人参皂苷Rg1抗血管内膜增生与其抗氧化和上调eNOS表达作用的关系[J].中国药理学通报,2012,28(3):388-392,5.基金项目
贵州省优秀科技教育人才省长专项基金项目[No黔省合字(2007)53号] （2007）
贵州省科技厅联合基金[黔科合J字LKZ(2010)31号] （2010）
