中国药理学通报2012，Vol.28Issue(3)：392-396,5.DOI:10.3969/j.issn.1001-1978.2012.03.022
芝麻素对2型糖尿病大鼠主动脉内皮功能的保护作用
Protective effect of Sesamin on endothelial function of type-2 diabetic rats
摘要
Abstract
Aim To study the ameliorative effect of sesamin ( Ses ) on endothelial dysfunction in diabetic rats and related mechanism. Methods Diabetic rats were induced by low dose streptozotocin( STZ ) and fed with high-fat diet. Then, rats were randomly divided into model group and Ses( 120, 60 mg · kg-1 ·d-1, respectively ig, for 8 weeks. ) groups. In addition, control group was set up. Eight weeks later, rats were sacrificed, the malondialdehyde ( MDA ) content, total antioxidative capacity( T-AOC ) in serum were tested, and the protein expression of endothelial nitric oxide synthase ( eNOS ), nitrotyrosine ( NT ) and nicotinam-ide adenine dinucleotide phosphate( NADPH ) oxidase subunit P47phox in aortas were deteced by Western blotting. In addition, vasorelaxation response to acetylcho-line( Ach ), and functional assessment of nitric oxide ( NO ) bioactivity were also determined in aortic rings. Results Compared with model group, vasodilation response of Ses (120 mg · kg-1 ) group was significantly improved. The MDA content was markedly decreased, T-AOC and NO level were increased. The protein expression of eNOS was significantly increased , while NT and P47phox were significantly decreased. Conclusion Ses can improve endothelial dysfunction through alleviated oxidative stress, enhance NO bioavailability by up-regulating eNOS expression and down-regulating NT and P47phox expression.关键词
芝麻素/糖尿病/内皮功能损伤/内皮型一氧化氮合酶/硝基酪氨酸/NADPH氧化酶/氧化应激Key words
sesamin/ diabetes/ endothelial dysfunction/ endothelial nitric oxide synthase/ nitrotyrosine/ NADPH oxidase/ oxidative stress分类
医药卫生引用本文复制引用
郭莉群,杨解人,孔祥..芝麻素对2型糖尿病大鼠主动脉内皮功能的保护作用[J].中国药理学通报,2012,28(3):392-396,5.基金项目
安徽省高校省级自然科学研究项目(No KJ2009B017) （No KJ2009B017）
安徽省高等学校省级优秀青年人才基金项目(No 2009SQR184) （No 2009SQR184）
