中国药理学通报2012，Vol.28Issue(3)：402-406,5.DOI:10.3969/j.issn.1001-1978.2012.03.024
针刺对海洛因成瘾戒断大鼠下丘脑-垂体-肾上腺系统的影响
Effect of acupuncture on hypothalamic-pituitary-adrenalsystem in heroin withdrawal rats
摘要
Abstract
Aim To assess the effect of acupuncture on anxiety-related behaviors after heroin-withdrawal, and explore its possible mechanism. Methods Heroin was injected subcutaneously according to the principle of daily increasing dose in rats of experimented group. The abstinence signs were precipitated by naloxone after drugs were stopped, and the animal model of heroin addiction was established. Acupuncture was continuously given for 7 days. The elevated plus maze test was used to observe anxiety level after heroin-withdrawal and the anti-anxiety effect of acupuncture. The concentration of corticosterone ( CORT ), adrenocortico-tropic hormone ( ACTH ) and arginine vasopressin ( AVP ) in the plasma of the rats were detected by radio-immunity. Expressions of Fos protein in the para-ventricular nucleus of rat hypothalamus were detected by immunohistochemistry method. Results Acupuncture significantly increased the percentage of entries in-to open arms and time spent on open arms( P <0. 01 or P < 0. 05 ). The radioimmunoassay results showed that acupuncture decreased plasma CORT, ACTH and AVP in heroin-withdrawal rats( P < 0. 01 or P < 0. 05 ). The immunohistochemistry results showed that the expression of Fos protein in hypothalamic paraventricular nucleus was decreased after acupuncture ( P < 0. 01 or P < 0. 05 ). Conclusions Acupuncture reduces anxiety level of heroin-withdrawal rats and modulates the hypothalamic-pituitary-adrenal system activities. These findings suggest that acupuncture at "shenmen" point may be useful as a therapeutic treatment in heroin withdrawal induced emotional disorders, and its mechanism may be related to modulating the activities of hypothalamic-pituitary-adrenal system.关键词
针刺/海洛因成瘾戒断/"神门"穴/焦虑相关症状/下丘脑-垂体-肾上腺系统/室旁核/Fos蛋白Key words
acupuncture/ heroin-withdrawal/ " shen men" point/ anxiety-related symptoms/ hypothalamic-pituitary-adrenal/ paraventricular nucleus/ Fos protein分类
医药卫生引用本文复制引用
王桂华,潘贵书,郭莉,韩艳春..针刺对海洛因成瘾戒断大鼠下丘脑-垂体-肾上腺系统的影响[J].中国药理学通报,2012,28(3):402-406,5.基金项目
贵州省教育厅资助项目(No TZJF2006年32号) （No TZJF2006年32号）
