中国药理学通报2012，Vol.28Issue(3)：421-424,4.DOI:10.3969/j.issn.1001-1978.2012.03.028
蓝萼甲素对宫颈癌HeLa细胞的作用及其相关机制研究
Effects of Glaucocalyxin A on HeLa cell and its molecular mechanism
摘要
Abstract
Aim To investigate the role of mitochondrial aldehyde dehydrogenase 2 ( ALDH2 ) in different stages of rat diabetic cardiomyopathy ( DM ) and analyze the related mechanism. Methods Diabetic model in SD rat was induced by a single intraperitoneal injection of streptozoticin ( STZ ). Hearts isolated from DM 4 wk,8 wk,12 wk rats were perfused on a langendorff apparatus. The ventricular hemodynamic parameters, SOD activity, MDA and hydroxyproline ( Hp ) contents were measured. The expression of ALDH2 at mRNA level of left anterior myocardium was detected by Real-Time qPCR analysis. Results In contrast to normal group, there were no particular changes of left ventricular developed pressure and maximal rise/fall rate of left ventricular pressure in DM 4 wk group, but MDA and Hp contents were increased, SOD activity and the expression of ALDH2 mRNA level were reduced. InDM 8 wk and DM 12 wk group, there were significant decreases of left ventricular developed pressure, maximal rise/fall rate of left ventricular pressure and SOD activity, MDA and Hp contents were increased. The expression of ALDH2 mRNA level was reduced. With the diabetic cardiomyopathy development, the ventricular hemodynamic parameters, SOD activity and AL-DH2 mRNA level were further reduced, and MDA, Hp contents were increased. Conclusion With the development of diabetes, the expression of heart mitochondrial ALDH2 is further decreased, which may be related to the decrease of antioxidant ability, and the aggravation of cardiac fibrosis.关键词
蓝萼甲素/宫颈癌HeLa细胞/Bcl-2/Bax/Caspase-3/Caspase-9/细胞凋亡Key words
diabetic cardiomyopathy/ mitochondrial ALDH2/ real-time qPCR/ super oxidase dimutase/ malondialdehyde/ hydroxyproline分类
医药卫生引用本文复制引用
黄敬敬,王修珍,蒋小岗,顾振纶,郭次仪..蓝萼甲素对宫颈癌HeLa细胞的作用及其相关机制研究[J].中国药理学通报,2012,28(3):421-424,4.基金项目
香港保健协会研究资助项目(No 20080908-4HK) （No 20080908-4HK）
