中国药理学通报2012，Vol.28Issue(3)：435-438,4.DOI:10.3969/j.issn.1001-1978.2012.03.031
体外培养单个造血干细胞实验方法的建立
Establishment of experimental methods to culture singlehematopoietic stem cell in vitro
摘要
Abstract
Aim To explore the effect of nomegestrol acetate ( NOMAc ) on the apoptosis of endometriosis ( Ems ) in rat models and its mechanism. Methods The rat models of NOMAc were established by surgical operation method; histopathologic changes of ectopic endometrium were tested by HE staining; the level of antiendometrium antibody ( EMAb ) was tested by Elisa; the expression levels of apoptosis factors were tested by Western blot. Results NOMAc ( 0. 5,1. 5, 15 mg ·kg-1 ) shriveled ectopic endometrium; the number of endometrial gland was fewer; glandular cavity was smaller in NOMAc groups than those of modelgroups. NOMAc reduced dose-dependently the endometrial thickness and the level of EMAb. NOMAc( 0. 5, 1.5,15 mg · kg-1 ) increased Caspase-9, Caspase-3 and Bax protein expression and reduced Bcl-2 protein expression. Conclusion The inhibitory effect of NOMAc on ectopic endometrium of rat models may be related to reducing the level of EMAb, increasing Bax, reducing Bcl-2 protein expression, and activating Caspase-9 and Caspase-3 protein expression in endogenous apoptosis pathway.关键词
单个HSC培养/流式细胞分选与分析/血细胞涂片/p18/小分子/干细胞扩增Key words
nomegestrol acetate/ endometriosis/ apoptosis / Bcl-2/ Bax/ Caspase-9/ Caspase-3分类
医药卫生引用本文复制引用
李乔,高瀛岱,纪庆,王金宏,许静,田晨,程辉,刘延风,张娜,程涛..体外培养单个造血干细胞实验方法的建立[J].中国药理学通报,2012,28(3):435-438,4.基金项目
国家自然科学基金资助项目(No 81170465,90913018,81090411) （No 81170465,90913018,81090411）
实验血液学国家重点实验室开放课题基金(ZK11-07,ZK08-04) （ZK11-07,ZK08-04）
