中国药理学与毒理学杂志2012，Vol.26Issue(1)：25-29,5.DOI:10.3867/j.issn.1000-3002.2012.01.006
冷水泳浴对高尿酸血症模型大鼠诱发痛风性关节炎的影响
Effect of swimming in cold water on gouty arthritis of rats with hyperuricemia
摘要
Abstract
OBJECTIVE To investigate the effect of swimming in cold water to prepareing a gouty arthritis model with clinical pathogenesis. METHODS A persistant hyperuricemic model was prepared by giving rats oxonic acid 1 ml·kg-1 and feeding them with hypoxanthine. Rats swam in cold water 10 - 12℃ , 10 min every day, for 12 d during the model preparation. The rat hind limbs were observed every day to find out whether swelling occurred, and the number of rats with swelled hind limbs was recorded. Rat foot microcirculation was expressed as blood perfusion unit ( BPU) , which was determined by Doppler microcirculation radiometer. Uric acid concentrations in serum and joint cavity were determined by automatic biochemistry analyzer. The histopathological changes in hind ankle joints were observed with HE staining. RESULTS The incidence of swelling of hind limbs of rats in hyperuricemia + swimming in cold water group was 76. 7% , but no swelling was observed in other groups. Rat foot microcirculation was influenced obviously by swimming in cold water and hyperuricemia. Compared with normal group, BPU in swimming in cold water, hyperuricemia model and hyperuricemia + swimming in cold water groups significantly decreased ( P < 0.01). The microcirculation in hyperuricemia + swimming in cold water group was slower than that in swimming in cold water and hyperuricemia model groups (P <0. 01 ). On the 6th and 12th day after prepariation of the model, compared with normal group (80 ±13) and (81 ±41 ) |xmol·L-1, serum uric acid in swimming in cold water group did not change. Serum uric acid was (550 ±362) and (1073 ±332) μmol ? L-1 in hyperuricemia model group and (570 ± 458) and (817 ± 338 ) μmol ? L -1 in hyperuricemia + swimming in cold water group, which were all obviously higher than those in normal group (P<0.01). Uric acid concentration in joint cavity in three treatment groups were all higher than that normal group (18 ±16) μmol·L-1 , and uric acid in hyperuricemia + swimming in cold water group (382 ±200) μmol·L-1 was much higher than that in swimming in cold water group (26 ± 12) μmol·L-1 and hyperuricemia model group (137 ±53 ) μmol·L-1 ( P < 0. 01) . The histological abnormalities of ankles showed that rats in hyperuricemia + swimming in cold water group suffered from ankle injury, such as hemangiectasis, congestion, and infiltration of inflammatory cells in synovium and the soft tissue around, while the other groups did not. CONCLUSION Swimming in cold water can induce swelling similar to gouty arthritis with clinical characteristics in persistent hyperuricemia rats.关键词
模型,动物/关节炎,痛风性/高尿酸血症/低温Key words
models, animal/arthritis, gouty/hyperuricemia/low temperature分类
医药卫生引用本文复制引用
时乐,徐立,尹莲,曾凡伟,梅琦..冷水泳浴对高尿酸血症模型大鼠诱发痛风性关节炎的影响[J].中国药理学与毒理学杂志,2012,26(1):25-29,5.基金项目
江苏省中医药科技项目(LZ11195) （LZ11195）
