中国药理学与毒理学杂志2012，Vol.26Issue(1)：41-46,6.DOI:10.3867/j.issn.1000-3002.2012.01.009
复方银杏叶制剂对酒精性肝损伤的防护作用及机制
Protective effect of compound Ginkgo biloba on alcohol-induced liver injury and its mechanism
摘要
Abstract
OBJECTIVE To observe the protective effect of compound Ginkgo biloba ( CGB) against alcohol-mediated hepatic injury and its mechanism. METHODS Rats were randomized into seven groups; normal control, model, Ginkgo biloba extract (GBE) 0.8 g'kg~ , CGB 2.4, 0. 8 and 0.4 g·kg-1 , bifendate ( Bif) 0. 15 g·kg-1 groups. The alcohol-injured hepatic injury model was established after rats were ig given liquor-corn oil suspension for 10 weeks. And CGB, GBE and Bif were ig given in the meantime except normal and model control groups. The content of alamine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum and that of malondialde-hyde ( MDA ) , superoxide dismutase ( SOD ) , glutathione ( GSH ) in liver homogenate were measured , so were the hepatic histological and electron microscopy changes in liver mitochondria. Plasma concentrations of chlorzoxazone and DDS were tested before and after CGB was given by the method of " cocktail" , and their pharmacokinetic parameters were calculated to evaluate metabolism activities of cytochrome P4502E1, CYP2E1 and CYP3A4 indirectly. RESULTS Liver cells were severely damaged by alcohol, resulting in alcoholic hepatitis and the fat liver. Compared with normal control group, the activities of ALT, AST and MDA increased (P <0. 01) while GSH and SOD decreased (P < 0. 01). Compared with model group, ALT, AST and MDA obviously decreased (P <0. 05 , P <0. 01 ) in CGB 2. 4 and 0. 8 g·kg-1 groups, while GSH and SOD were significantly increased(P <0. 05 , P <0. 01 ) . AUC0_24 and cmax for chlorzoxazone and DDS after CGB was given significantly decreased (P <0. 05, P <0. 01 ) compared to those before CGB was given ig in model groups. CONCLUSION CGB may protect the liver from alcoholic injury and the mechanism may be related to antioxidation.关键词
银杏/植物提取物/酒精性肝疾病/细胞色素P450CYP2E1/细胞色素P450CYP3A4Key words
Ginkgo biloba/plant extracts/liver diseases, alcoholic/cytochrome P-450 CYP2E1/cytochrome P450 CYP3A4分类
医药卫生引用本文复制引用
潘苏华,刘平平,刘亚锋,高青..复方银杏叶制剂对酒精性肝损伤的防护作用及机制[J].中国药理学与毒理学杂志,2012,26(1):41-46,6.基金项目
江苏省中医局基金项目(H-024) （H-024）
