中国油料作物学报2011，Vol.33Issue(6)：533-539,7.
甘蓝型油菜隐性细胞核雄性不育上位抑制基因(esp)的分子标记开发
Molecular markers of esp : A recessive epistatic suppressor gene of recessive genic male sterile Brassica napus L.
摘要
Abstract
9012AB is a recessive epistatic genie male sterility (REGMS) two -type line of Brassica napus L. The sterility is controlled by two recessive duplicate genes ms3MS3ms4ms4 and one recessive epistatic inhibitor gene espesp. To effectively identify REGMS lines and their temporary maintainer lines, five AFLP ( amplified fragment length polymorphism) markers ( L1, L2, L3 , L4, L5 ) in couple to esp and two AFLP markers ( L6, L7) in repulsion to esp were identified through screening 1 024 primer combinations using 9012B and T45 segregation population. All the seven markers were located on one the same side of esp gene. Markers L6 and L7 co - segregated with Esp gene. Markers L1, L6 and L7 were successfully converted into SCAR ( sequence characterized amplified region) markers as SCL1, SCL2 and WSCL3, respectively. The accuracy rates of SCL1, SCL2 and WSCL3 were 90% , 100% and 100% , respectively. Result showed their potential in marker assisted selection of 9012AB.关键词
甘蓝型油菜/隐性核不育/上位抑制基因(esp)/分子标记Key words
Brassica napus/ Recessive genie male sterility/ Epistatic inhibitor gene (esp) / Moleculat marker分类
农业科技引用本文复制引用
李婧婧,谢彦周,洪登峰,刘平武,何庆彪,杨光圣..甘蓝型油菜隐性细胞核雄性不育上位抑制基因(esp)的分子标记开发[J].中国油料作物学报,2011,33(6):533-539,7.基金项目
国家863计划(2011AA10A104) （2011AA10A104）
国家973计划(2007CB109006) （2007CB109006）
