中国油料作物学报2011，Vol.33Issue(6)：598-602,5.
胡麻枯萎病生防芽孢杆菌筛选及抑菌效果研究
Screening of biocontrol bacillus against Fusarium wilt and control effects in linseed
摘要
Abstract
Biological control microorganism was isolated from pathogenic soil of linseed. Inhibition rates to Fu-sarium oxysporum f. sp. Lini were evaluated using 151 fungus isolates from different provinces. A bacterial XJ2 -20 from 21 antagonistic Bacillus strains showed strong antagonistic effect on the 151 fungus isolates. Its highest inhibition rate was 64.5%. Fermentation filtrate of XJ2 -20 affected mycelium growth and spore bourgeoning. Its efficiency of control was as high as 56. 3% in pot experiment. Optimized fermentation of XJ2 -20 was under 29℃ and pH7. 2 with 50 to 100mL LB medium in 300mL - flask for 5d. Antagonistic substance of XJ2 -20 was extracted by 70% (NH4)2SO4 precipitation at pH7. The extracted crude protein inhibited pathogens by leading to mycelium rupture, distortion, and inhibiting bourgeoning of spores, although it was sensitive to heat and tryroteinase.关键词
胡麻/枯萎病/生防芽孢杆菌/防治效果/发酵滤液Key words
Linseed/ Fusarium wilt/ Bacillus/ Biocontrol effect/ Fermentation filtrate分类
农业科技引用本文复制引用
郭景旭,张辉,李子钦,包玉英..胡麻枯萎病生防芽孢杆菌筛选及抑菌效果研究[J].中国油料作物学报,2011,33(6):598-602,5.基金项目
国家胡麻产业技术体系项目(CARS-17-GW-6) （CARS-17-GW-6）
