中国医学科学院学报2011，Vol.33Issue(6)：606-610,5.DOI:10.3881/j.issn.1000-503X.2011.06.004
表皮生长因子影响人羊膜间充质干细胞迁移的机制
Effect of Epidermal Growth Factor on Migration of Human Amniotic Mesenchymal Stem Cells
摘要
Abstract
Objective To explore the mechanism via which the epidermal growth factor (EGF) affects the migration of human amnion-derived mesenchymal stem cells ( hAMSCs). Methods In vitro cultured hAMSCs were divided into control (untreated), EGF group, inhibitor AG1478 + EGF group, inhibitor LY294002 + EGF group, and inhibitor U0126 + EGF group. The migration ability of hAMSCs in each group was measured using Transwell chamber. The expressions of phosphorylated EGFR (P-EGFR) , phosphorylated AKT (P-AKT), and phosphorylated ERK1/2 (P-ERK1/2) as well as the expressions of metalloproteinase(MMP) -2 and MMP-9 were detected using Western blot analysis. The differentially expressed genes in the culture solutions in ECF groups and control group were analyzed with RNA-Seq technique. Results Cells in EGF group had significantly stronger migration ability than in control group ( P = 0. 0361) , inhibitor AG1478 + EGF group (P = 0. 0113), inhibitor LY294002 + EGF group ( P = 0. 0169), and inhibitor U0126 + EGF group (P = 0. 0293). EGF increased the phosphorylation levels of EGFR, AKT and ERK, and increased the expression of MMP-2. However, the increased expressions of P-AKT and P-ERK could be suppressed by AG1478 and LY294002. As shown by GO functional enrichment analysis and KEGG pathway analysis, EGF increased the transcription of genes, which were mainly involved in transcriptional regulation, protein modification, and apoptosis inhibition. Genes that were involved in the MARK pathway included DUSP5, IL1B, DUSP6, NGF, and HSPA2. Conclusion EGF-induced migration of hAMSCs may be mediated by the signaling pathways of PI3K and ERK, which needs MMP-2 expression and the co-expression of genes involved in transcriptional regulation, protein modification, and apoptosis inhibition.关键词
人羊膜间充质干细胞/表皮生长因子/磷脂酰肌醇激酶-3/细胞外调节蛋白激酶Key words
human amniotic mesenchymal stem cells/epidermal growth/actor/phosphatidylinositol 3-kinases/extracellular regulated protein kinases分类
医药卫生引用本文复制引用
李彩虹,施萍,庞希宁..表皮生长因子影响人羊膜间充质干细胞迁移的机制[J].中国医学科学院学报,2011,33(6):606-610,5.基金项目
沈阳市科学技术计划(090063、F10-222-4-00) （090063、F10-222-4-00）
