中国医学科学院学报2011，Vol.33Issue(6)：611-614,4.DOI:10.3881/j.issn.1000-503X.2011.06.005
人羊膜负载人羊膜间充质干细胞对SD大鼠皮肤创面愈合的影响
Effect of the Human Amniotic Membrane Loaded with Human Amniotic Mesenchymal Stem Cells on the Skin Wounds of SD Rats
摘要
Abstract
Objective To observe the effect of the human amniotic membrane ( HAM ) loaded with human amniotic mesenchymal stem cells (hAMSCs) on the skin wounds of SD rats. Methods The amniotic epithelial cells were removed by trypsin digestion. hAMSCs were loaded onto HAM and then covered on rats' skin defects. The wound healing was observed by HE staining and immunohistoehemistry, and the results were compared with the amniotic membrane group and blank control group. Results The average wound healing time was ( 18. 3 ± 0. 9) d in the HAM load with hAMSCs group, which was significantly faster than those in the blank control group [ (26. 4 ±0.7) d, P < 0. 01 ] and the amniotic membrane group[(21.5±1.2)d,P<0. 05]. After 11 d and 14 d, the wound healing rates in the HAM load with hAM-SCs group were (81. 5 ±7. 2)% and (94. 3 ±3. 6)% , respectively, which were significantly higher than those in the blank control group [ ( 48. 5 ±3.2)% and ( 74. 3 ± 4. 3 ) % ] and the amniotic membrane group [ (68. 5 ± 4. 5) % and (86. 8 ± 4. 8 ) % ] ( all P < 0. 01 ). Skin biopsy/HE staining confirmed that the quality of wound healing in the HAM load with hAMSCs group was significantly better than in the amniotic membrane group and the blank control group. Immunohistochemical staining showed that the number of CK19-positive epidermal stem cells in the HAM load with hAMSCs group (48. 2 ± 3. 2) was significantly larger than those in the amniotic membrane group (37. 7 ± 3. 1 ) ( P < 0. 05 ) and the blank control group (29. 6 ±2. 4) ( P < 0. 01). Furthermore, the vascular endothelial growth factor expression (64. 5 ±4. 5) in the HAM load with hAMSCs group was also significantly higher than those in the amniotic membrane group (52. 6 ±3. 8) (P<0. 05) and the blank control group (40. 7 ±3. 1) (P <0. 01). Conclusion HAM loaded with hAMSCs may promote the repair of skin wounds by promoting the regeneration of epidermal stem cells and capillaries.关键词
人羊膜/人羊膜间充质干细胞/创伤修复/角蛋白19/血管内皮生长因子Key words
human amniotic membrane/human amniotic mesenchymal stem cells/wound healing/cytokeratinl9/vascular endothelial growth factor分类
医药卫生引用本文复制引用
霍双枝,施萍,庞希宁..人羊膜负载人羊膜间充质干细胞对SD大鼠皮肤创面愈合的影响[J].中国医学科学院学报,2011,33(6):611-614,4.基金项目
沈阳市科学技术计划(090063、 F10-222-4-00) （090063、 F10-222-4-00）
