中国医学科学院学报2011，Vol.33Issue(6)：620-623,4.DOI:10.3881/j.issn.1000-503X.2011.06.007
小鼠大脑皮层层次特异表达基因天然反义转录物的筛选与鉴定
Screening and Identification of Natural Antisense Transcript in Mouse Cerebral Cortex
摘要
Abstract
Objective To screen and identify the possible existence of natural antisense transcript ( NAT) within the mouse neocortex. Methods Sixty-three cerebral cortex layer-specific genes were screened by bioinformatics prediction in mice, among which 31 mice with potential N Ats were screened. NAT was identified using reverse transcription polymerase chain reaction ( RT-PCR) and then cloned in pGEM-T Vector System for sequencing. Results Among 31 genes predicted using bioinformatics, 8 were proved to be NAT positive by RT-PCR. Conclusions NATs exist in the mouse neocortex tissue during the development of cerebral cortex. NATs may influence mouse cortical development by regulating the related coding genes.关键词
天然反义转录物/小鼠大脑皮层/筛选/鉴定Key words
natural antisense transcript/mouse cerebral cortex/screening/identification分类
医药卫生引用本文复制引用
李萍,张靖,李爱花,王珊,谈小超,阴彬,彭小忠..小鼠大脑皮层层次特异表达基因天然反义转录物的筛选与鉴定[J].中国医学科学院学报,2011,33(6):620-623,4.基金项目
国家自然科学基金(31071203) （31071203）
