中国医学科学院学报2011，Vol.33Issue(6)：629-631,3.DOI:10.3881/j.issn.1000-503X.2011.06.009
小鼠睾丸曲细精管生精上皮的分离及形态观察
Separation and Morphological Observation of Mouse Testis Seminiferous Tubule Epithelium
摘要
Abstract
Objective To explore a simple and feasible technique to locate proteins during spermato-genesis. Methods Various germ cells and somatic cells were separated by collagenase I and Dnase I after the albuginea was removed. The cells were then smeared, dyed, and observed directly under fluorescence and con-focal microscopy. Results Germ cells at different steps were successfully identified by specific dyestuffs for acrosome and nucleolus. Conclusion A simple method for locating proteins during permatogenesis was successfully developed.关键词
精子发生/顶体形成/细胞核Key words
spermatogenesis/acrosome formation/nucleolus分类
生物科学引用本文复制引用
付俊,缪时英..小鼠睾丸曲细精管生精上皮的分离及形态观察[J].中国医学科学院学报,2011,33(6):629-631,3.基金项目
国家重大研究计划项目(2011CB944302)和国家重点实验室专项基金(2060204) （2011CB944302）
