中国医学科学院学报2011，Vol.33Issue(6)：644-648,5.DOI:10.3881/j.issn.1000-503X.2011.06.012
大量制备过继免疫治疗用γδT细胞时培养基的选择
Selection of Culture Media for the Mass Production of γδT Cells Used in Adoptive Immunotherapy
摘要
Abstract
Objective To select the optimal culture media for the mass production of 78T cells used in adoptive immunotherapy. Methods Three different culture media (RPMI-1640, AIM-V, and OpTmizer, with or without autologous serum) were used to culture γδ8T cells. The survival rate, purity, proliferation efficiency , and biological functions of the expanded γδT cells were examined and compared. Results The survival rate of γδT cells expanded in RPMI-1640 decreased over culture time. The purities of γδT cells cultured in AIM-V or OpTmizer with or without serum were higher than those cultured in RPMI-1640. After two weeks of culture in the absence of serum, the purity and proliferation efficiency of γδ8T cells cultured in OpTmizer were significantly higher than those cultured in RPMI-1640 ( P < 0. 05) and AIM-V ( P < 0. 05 ). γδT cells in different culture media had similar CD107a expression and tumor necrosis factor-a production ( P > 0. 05 ) .However, cells expanded in RPMI-1640 exhibited significantly weaker cytotoxicity against Daudi lymphoma cells than those expanded in OpTmizer ( P < 0. 05) and AIM-V (P < 0. 05). Conclusion Due to low serum-dependence , high proliferation efficiency, and favorable biology function of expanded cells, OpTmizer is the most suitable medium for the mass production of γδT cells used in adoptive immunotherapy.关键词
γδT细胞/过继免疫治疗/培养基Key words
γδT cells/adoptive immunotherapy/culture medium分类
医药卫生引用本文复制引用
周建华,康宁,崔莲仙,何维..大量制备过继免疫治疗用γδT细胞时培养基的选择[J].中国医学科学院学报,2011,33(6):644-648,5.基金项目
国家高技术研究发展计划项目(863项目)(2006AA02Z480)和国家自然科学基金(30972776) （863项目）
