中国医学装备2011，Vol.8Issue(10)：35-37,3.
制作血液涂片的技术改进
Improvement in techniques of making blood smear slides
陈学军 1刘孝武 2王英1
作者信息
- 1. 河北省邯郸市中医院检验科,河北邯郸056001
- 2. 河北工程大学医学院临检教研室,河北邯郸056001
- 折叠
摘要
Abstract
Objective：To improve the traditional method of making blood smear slides to standardize the new method to effectively control the quality of blood tests.Methods：Using push-slides at an fixed angle of different degrees from 10° to 30° to make blood smear slides with a certain amount of EDTA anticoagulated blood.Under a microscope,the length of the blood membranes and the evenness of the distribution of cells were observed after staining the smear slides.Then measurement and comparison were performed to find out those smear slides which conform to established standards and identify the standard angles of push-slides.The standard blood smear slides were made using push-slides at the standard angles.The standard blood smear slides were compared with those made by the traditional method.Results：The blood smear slides were made with 5uL of blood using push-slides at an fixed angle of 17°/18° degree.The X and S values of membrane lengths for the slides were 4.081/3.987 and 0.015/0.0103,respectively.The X and S values for the areas of evenly distributed cells for the slides were 3.033/2.971 and 0.015/0.0104,respectively.The P value for the comparison between the slides by the traditional method and those by using push-slides at a fixed angle of 17° was less than 0.001,which means there is significant difference between these 2 kinds of slides.Conclusion：Blood smear slides made by using push-slides at a fixed angle of 17° or 18° with 5uL of blood satisfies the requirements for standard blood smear slides,and are therefore helpful in controlling the quality of morphological examination of blood cells,thus increasing the accuracy of diagnosis.关键词
血液涂片/固定角度/标准化/改进Key words
Blood smear slide/Fixed angle/Standardization/Improvement分类
医药卫生引用本文复制引用
陈学军,刘孝武,王英..制作血液涂片的技术改进[J].中国医学装备,2011,8(10):35-37,3.
