中国药业2012，Vol.21Issue(5)：13-14,2.
反相高效液相色谱法测定家兔体内乙酰半胱氨酸血药浓度
Determination of Acetylcysteine in Rabbit Plasma by RP-HPLC
摘要
Abstract
Objective To establish a method for acetylcysteine in rabbit plasma by RP-HPLC.Methods The samples were separatedon an Eclipse XDB-C18(150mm×4.6mm,5μm),using a mobile phase of 0.01 mol/L phosphoric acid and methanol(47:53)at 30℃,the flow rate was 1.0mL/min,the detection wavelength was set at 257 nm.Results the recoveries of the control samples oflow, medium and high conceatrations of precisions, the intraday RSDs were 0.90%,1.36% and 2.15%;and the interday RSDs were1.15%, 2.31% and 4.98%.The standard curve was linear ocer the range of 4-80 μg/Ml concentrations of the acclylcysreine(r2=0.9997).Couclusion This method is simple, accurate, reproducible,andsuitable for the detemination of acelysieine in rabbitplasma.关键词
乙酰半胱氨酸/血药浓度/反相高效液相色谱法Key words
acelylcyeleine/ plaama concentralion/ RP-HPLC分类
医药卫生引用本文复制引用
席建军,王福根,庄让笑,缪樑斌..反相高效液相色谱法测定家兔体内乙酰半胱氨酸血药浓度[J].中国药业,2012,21(5):13-14,2.基金项目
浙江省卫生厅2009年重点资助项目,项目编号:2009A170. （）
