摘要
Abstract
Objective To establish a method for monammonium glycyrrhizinate in hare plasma by RP - HPLC. Methods The plasma samples were collected after monoammonium glycyrrhizininate injection were injected, at 0. 5,1,2,3,4, 8,10,12,18,24 h,blood samples were centrifuged within 30 minutes. Methanol was added to precipitate the proteins. 20 μL of the supernatant liquid was injected into the HPLC system. The samples were separated on a Eclipse XDB -C18(150 mm ×4. 6 mm, 5 μm), Using a mobile phase of 0. 01 mol/L phosphoric acid and methanol(68 : 32). At 30 ℃, the flow rate was 1 mL/min,the detection wavelength was 257 nm. Results The recoveries of the control samples of low,medium and high concentrations of precisions,the intraday RSD were 0.93% ,2. 15% and 3.66%; and the interday RSD were 1.33% ,3.90% and 5. 11%. The standard curve was linear over the range of 5 - 1 000 mg/L concentrations of the monammonium glycyrrhizinate ( r2 =0. 999 6)and the Cmax = (470. 3 ± 85. 9)mg/L, Tmax = (30. 1 ± 16. 2)min, AUG0-∞ = (95 752.4 ±5 781. 5)mg/(L ? Min) CLz = (0. 000 8 ±0.000 2)L/(min ? Kg), Vz = (0. 19 ±0. 03)L/kg, T1/2 = (145. 3 ±35. 3)min. Conclusion This method is simple, accurate, reproducible, and suitable for the determination of monammonium glycyrrhizinate in hare plasma.关键词
甘草酸单铵/药代动力学/反相高效液相色谱法Key words
monammonium glycyrrhizinate/ Pharmacokinetics/ RP - HPLC分类
医药卫生
