中国药业2012，Vol.21Issue(6)：42-43,2.
高效液相色谱法测定盐酸左旋多巴异戊酯的含量
Determination of L-Dopa Isopentyl Ester Hydrochloride by HPLC
许崇摇 1蒋伟哲 1吕聪 1吕立 1邱卓 1黄静1
作者信息
摘要
Abstract
Objective To establish a high performance liquid chromatography (HPLC) method for the content determination of levodopa isopentyl ester hydrochloride. Methods The Ultimate C18 column (250 mm×4.6 mm, 5 μm) was used and the mobile phase was acetonitrile was 0. 05 mol/L potassium dihydrogen phosphate (adjusting pH 3.5 with phosphoric acid, 70 : 30), the flow rate was 1.0 mL/min. The detection wavelength was 280 nm. The column temperature was 30 t and the injection volume was 20 μL. Results All the related substances gained a completely chromatographic separation with the principal peak. A good linear relationship was observed in the concentration range of 16. 1 - 322. 0 μg/mL, with the correlation coefficient of 0. 999 9( n = 6). The average recovery rate was 98.41% and RSD was 1. 85% (n = 6). Conclusion The method is proved to be accurate and sensitive with high precision and strong specificity, which can be applicable for the content assay of levodopa isopentyl ester hydrochloride.关键词
高效液相色谱法/盐酸左旋多巴异戊酯/含量测定Key words
HPLC/ L - dopa isopentyl ester hydrochloride/ content determination分类
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许崇摇,蒋伟哲,吕聪,吕立,邱卓,黄静..高效液相色谱法测定盐酸左旋多巴异戊酯的含量[J].中国药业,2012,21(6):42-43,2.
