中国肿瘤生物治疗杂志2011，Vol.18Issue(6)：653-657,5.DOI:10.3872/j.issn.1007-385X.2011.06.012
人血管能抑素抑制小鼠Lewis肺癌移植瘤生长、转移及血管新生
Canstatin inhibits growth, metastasis and angiogenesis of transplanted Lewis lung cancer in mice
陆卫忠 1肖飞 1黄桂君 2李玉英 2李瑾 2陈维中 2钱桂生2
作者信息
- 1. 解放军303医院呼吸内科,广西南宁530021
- 2. 第三军医大学新桥医院全军呼吸内科研究所,重庆400037
- 折叠
摘要
Abstract
Objective: To investigate the effect of canstatin on growth, metastasis and angiogenesis of transplanted Lewis lung cancer in mice. Methods: The recombinant pCMV-Script/canstatin vector or the empty vector was transfected into A549 cells by electroporation, and the positive clones were screened with G418. The expressions of canstatin mRNA and protein in transfected A549 cells were examined by RT-PCR and Western blotting, respectively. Furthermore, transplanted Lewis lung cancer mouse model was established, and therapeutic effect of supernatant of pCMV-Script/canstatin transfected A549 cells on transplanted Lewis lung cancer was observed. Microvessel density of transplanted tumors in different therapy groups was observed by immunohistochemistry. Results: Positive clones of A549 cells transfected with pC-MV-Script/canstatin were successfully obtained by G418 screening, and could effectively express canstatin mRNA and protein. The tumor size of the pCMV-Script/canstatin group ( 1. 47 ±0. 21 cm3 ) was significantly smaller than that in the pCMV-Scrip group (2.43 ± 0. 15 cm3 ) and NS group (2. 53 ± 0. 18 cm3 ) ( P < 0. 01) . The number of pulmonary meta-static nodes was 3. 00 ± 1. 00, 7. 80 ± 1. 48 and 7. 60 ± 2. 41 respectively for pCMV-Script/canstatin, pCMV-Script and NS groups, so pCMV-Script/canstatin significantly inhibited metastasis of tumors (P <0. 01) . The amount of microvessel count ( MVC) in pCMV-Script/canstatin group was markedly decreased compared to that of pCMV-Script and NS groups (P < 0. 01) . Conclusion: The pCMV-Script/canstatin mediates expression of canstatin in A549 cells. Canstatin in A549 supernantant has a strong inhibitory effect on growth, metastasis and angiogenesis of Lewis lung carcinoma.关键词
人血管能抑素/转染/血管生成/Lewis肺癌Key words
canstatin/ transfection angiogenesis/ Lewis lung carcinoma分类
医药卫生引用本文复制引用
陆卫忠,肖飞,黄桂君,李玉英,李瑾,陈维中,钱桂生..人血管能抑素抑制小鼠Lewis肺癌移植瘤生长、转移及血管新生[J].中国肿瘤生物治疗杂志,2011,18(6):653-657,5.
