中国中医药信息杂志2011，Vol.18Issue(12)：59-61,3.DOI:10.3969/j.issn.1005-5304.2011.12.023
20(S)-原人参二醇油水分配系数测定和大鼠在体肠吸收的研究
Apparent Oil/water Partition Coefficient of 20(S)-PPD and Its Intestinal Absorption in Rats
摘要
Abstract
Objective To determine the apparent oil/water partition coefficient of 20(S)-PPD and to investigate its absorption mechanism by in situ perfusion method in rat intestines. Methods The concentration of 20(S)-PPD was determined by HPLC. A shake flask method was established to determine oil/water partition coefficient of 20(S)-PPD. In situ perfused rat intestinal model was used to calculate the kinetic parameters by weight methods. Results At 37 ℃, the log Papp of 20(S)-PPD in octanol/water system was 1.72. Log Papp in different phosphate buffer solutions were close to that in aqueous phase. 20(S)-PPD was well absorbed in general intestinal tract, where its absorption rate constant (Ka) was duodenum>jejunum>ileum>colon. Ka and Papp in rat colon was relatively lower than in other intestinal tracts. It implied that the main absorption segment of 20(S)-PPD was small intestine. Conclusion The pH of buffer solution had no influence on its distribution coefficient. 20(S)-PPD was well absorbed in general intestinal tract with small intestine as its main absorption segment. It suggested that its stay in small intestine should be prolonged to promote its bioavailability in the relevant new-drug development.关键词
20 (S) -原人参二醇/表观油水分配系数/在体肠吸收/高效液相色谱法Key words
20(S)-protopanaxadiol/ apparent oil/water partition coefficient/ in situ intestine absorption/ HPLC分类
医药卫生引用本文复制引用
王冰,王玉芹,浦益琼,徐本亮,陶建生,张彤..20(S)-原人参二醇油水分配系数测定和大鼠在体肠吸收的研究[J].中国中医药信息杂志,2011,18(12):59-61,3.基金项目
上海市科委纳米专项(1052nm05200) （1052nm05200）
上海市教委预算内科研项目(2010JW22) （2010JW22）
