中国中医药信息杂志2012，Vol.19Issue(1)：39-41,3.DOI:10.3969/j.issn.1005-5304.2012.01.014
丹参酮ⅡA对乏氧环境下人高转移性巨细胞肺癌细胞表面β1-整合素及E-cadherin表达的影响
Effect of Tanshinon Ⅱ A on Expression of β1-integrin and E-cadherin on the Surface of Cell PG Cultured in Hypoxic Environment
摘要
Abstract
Objective To investigate the effect of Tanshinon ⅡA on the expression of β1-integrin and E-cadherin on the surface of cell PG cultured during normoxia and hypoxia. Methods The revival cell PG was divided into three dosages (high, medium and low) group of Tanshinon II A, DDP group and negative control group. All of five groups were cultured separately in the normal and hypoxic environment. The expression of E-cadherin and β1-integrin on the surface of PG cell by the flow cytometry 48 h later. Results In the hypoxic environment, the fluorescence intensity of β1-integrin increased with the density of Tanshinon ⅡA but the expression of fluorescence intensity was fading gradually. However, the rate of positive cells of β1-integrin was almost 100%, with no statistical significance. The expression of E-cadherin was increased with the density of Tanshinon Ⅱ A. The amount of positive cell was rising, but the fluorescence intensity of E-cadherin was very weak, with no statistical significance. In the normal oxygen environment, the fluorescence intensity of pl-integrin increased with the density of Tanshinon ⅡA, but the rate of positive cells of β1-integrin was almost 100%, with no statistical significance. The expression of E-cadherin was increased with the density of Tanshinon Ⅱ A. The amount of positive cell was decreasing, but the fluorescence intensity of E-cadherin was very weak, with no statistical significance. Conclusion In the normoxia, Tanshinon ⅡA restrains the expression of E-cadherin and pi-integrin to inhibit the matastasis. In the hypoxic environment, Tanshinon ⅡA restrains the pi-integrin to inhibit the matastasis and promote the expression of E-cadherin to strengthen the ability of invasion and matastasis of cell PG.关键词
人高转移性巨细胞肺癌细胞/丹参酮ⅡA/β1-整合素/黏附分子Key words
PG cell/Tanshinon ⅡA/ pi-integrin/ adhesion moleeule分类
医药卫生引用本文复制引用
张明辉,张培彤,周天..丹参酮ⅡA对乏氧环境下人高转移性巨细胞肺癌细胞表面β1-整合素及E-cadherin表达的影响[J].中国中医药信息杂志,2012,19(1):39-41,3.基金项目
国家自然科学基金(30873334) （30873334）
