中国中医药信息杂志2012，Vol.19Issue(2)：55-57,3.DOI:10.3969/j.issn.1005-5304.2012.02.022
苍耳子配伍黄芪的HPLC-DAD指纹图谱研究
Study of Variation on Fingerprint Chromatogram of Compatibility Extract Derived from Fructus Xanthii and Radix Astragali by HPLC-DAD
摘要
Abstract
Objective To establish the method for analyzing compatibility components from Fructus Xanthii and Radix Astragali by HPLC fingerprint chromatogram and to study the influence of main characteristic peaks variations induced by compatibilities. Methods Diamonsil-Cis column (4.6 mmX250 mm, 5 pm) was used to analyse these samples including the water extracts of Fructus Xanthii, Radix Astragali and compatibilities between them, with methanol and water containing 0.2% methanoic acid as mobile phases in a gradient elution, detection wavelength as 260 nm, and flow rate was 0.8 mL/min. Results HPLC fingerprint chromatogram of the compatibilities from Fructus Xanthii and Radix Astragali was established, and the whole characteristic peaks were the summation, with no new characteristic peaks existing Compared with the single decoction, dissolution rate of some ingredients was decreased reciprocally in compatibilities. Conclusion The method is simple and convenient, which provides the references to clinical applications of compatibility and the basis for the quality assessment of compound.关键词
苍耳子/黄芪/配伍/指纹图谱/高效液相色谱法Key words
Fructus Xanthii/ Radix Astragali/ compatibility/ fingerprint/ HPLC分类
医药卫生引用本文复制引用
曹敏,武斌,刘树民..苍耳子配伍黄芪的HPLC-DAD指纹图谱研究[J].中国中医药信息杂志,2012,19(2):55-57,3.基金项目
国家自然科学基金(30873435) （30873435）
