首页|期刊导航|中华医学杂志（英文版）|Silencing of osteopontin promotes the radiosensitivity of breast cancer cells by reducing the expression of hypoxia inducible factor 1 and vascular endothelial growth factor
Silencing of osteopontin promotes the radiosensitivity of breast cancer cells by reducing the expression of hypoxia inducible factor 1 and vascular endothelial growth factor
YANG Li ZHAO Wei ZUO Wen-shu WEI Ling SONG Xian-rang WANG Xing-wu ZHENG Gang ZHENG Mei-zhu
中华医学杂志（英文版）2012，Vol.125Issue(2)：293-299,7.
中华医学杂志（英文版）2012，Vol.125Issue(2)：293-299,7.DOI:10.3760/cma.j.issn.0366-6999.2012.02.024
Silencing of osteopontin promotes the radiosensitivity of breast cancer cells by reducing the expression of hypoxia inducible factor 1 and vascular endothelial growth factor
Silencing of osteopontin promotes the radiosensitivity of breast cancer cells by reducing the expression of hypoxia inducible factor 1 and vascular endothelial growth factor
摘要
Abstract
Background Osteopontin (OPN) is a secreted phosphoglycoprotein (SSP) that is overexpressed in a variety of tumors and was regarded as a molecular marker of tumors.In this study,we intended to demonstrate the role of OPN in human breast cancer cell line MDA-MB-231.Methods Recombinant plasmid expressing small interfering RNA (siRNA) specific to OPN mRNA was transfected into MDA-MB-231 cells to generate the stable transfected cell line MDA-MB-343,and the empty plasmid tansfected cells (MDA-MB-neg) or wildtype MDA-MB-231 cells were used as control cells respectively.Expression of OPN,hypoxia inducible factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) proteins was analyzed by Western blotting analysis.The radiosensitivity of cells was determined by detecting cell apoptosis,cell proliferation and cell senescence.Results HIF-1 and VEGF proteins in MDA-MB-343 cells were significantly downregulated upon the efficient knockdown of OPN expression under either hypoxia or normoxia environment.Moreover,expression of OPN protein was upregualted upon hypoxic culture.Stable OPN-silencing also decreased cell invasion,increased cell apoptosis and cell senescence,as well as reduced clonogenic survival,resulting in increase radiation tolerance.Conclusions Suppression of OPN gene expression can enhance radiosensitivity and affect cell apoptosis in breast cancer cells.OPN seems to be an attractive target for the improvement of radiotherapy.关键词
breast cancer/osteopontin/small interfering RNA/radiosensitivityKey words
breast cancer/osteopontin/small interfering RNA/radiosensitivity引用本文复制引用
YANG Li,ZHAO Wei,ZUO Wen-shu,WEI Ling,SONG Xian-rang,WANG Xing-wu,ZHENG Gang,ZHENG Mei-zhu..Silencing of osteopontin promotes the radiosensitivity of breast cancer cells by reducing the expression of hypoxia inducible factor 1 and vascular endothelial growth factor[J].中华医学杂志（英文版）,2012,125(2):293-299,7.基金项目
This research was supported by a grant,from the Shandong Natural Foundation of China (No.Y2008C167). （No.Y2008C167）
