首页|期刊导航|中华医学杂志（英文版）|Ecological surveys of the Cryptococcus species complex in China

Ecological surveys of the Cryptococcus species complex in China

LI An-sheng CHEN Min SHI Wei-ming LIAO Wan-qing PAN Wei-hua WU Shao-xi Taguchi Hideaki GUO Ning-ru SHEN Yong-nian LU Gui-xia PAN Ru-gui ZHU Miao-chang

中华医学杂志（英文版）2012，Vol.125Issue(3)：511-516,6.

中华医学杂志（英文版）2012，Vol.125Issue(3)：511-516,6.DOI:10.3760/cma.j.issn.0366-6999.2012.03.020

Ecological surveys of the Cryptococcus species complex in China

LI An-sheng ¹CHEN Min ²SHI Wei-ming ³LIAO Wan-qing ²PAN Wei-hua ²WU Shao-xi ⁴Taguchi Hideaki ⁵GUO Ning-ru ⁴SHEN Yong-nian ⁴LU Gui-xia ⁴PAN Ru-gui ⁶ZHU Miao-chang⁷

作者信息

1. Institute of Dermatology, Chinese Academy of Medical Sciences,Nanjing, Jiangsu 210042, China(Li AS, Wu SX, Guo NR, Shen
2. Shanghai Key Laboratory of Molecular Medical Mycology,Institute of Dermatology and Mycosis of Changzheng Hospital,Second Military Medical University, Shanghai 200003, China
3. Department of Dermatology, First People's Hospital of Shanghai Jiao Tong University, Shanghai 200080, China
4. Institute of Dermatology, Chinese Academy of Medical Sciences,Nanjing, Jiangsu 210042, China
5. Medical Mycology Research Center, Chiba University, Chiba,Japan
6. Institute of Botany, Jiangsu Province and CAS, Nanjing, Jiangsu 210014, China
7. Jiangxi Guixi Forest Bureau, Guixi, Jiangxi 335400, China
折叠

摘要

Abstract

Background Despite recent reports on the molecular epidemiology of cryptococcal infections in China,clinical isolates have been mostly reported from human immunodeficiency virus (HIV)-negative patients,and environmental isolates from China have rarely been included.The aim of this study was to investigate the ecological profile of Cryptococcus (C.)neoformans and C.gattii in China.Methods A survey was performed in 10 cities from 20°N (North latitude) to 50°N and in a Eucalyptus (E.) camaldulensis forestry farm at the Guixi forestry center,China.Results Six hundred and twenty samples of pigeon droppings from 10 cities and 819 E.camaldulensis tree samples were collected and inoculated on caffeic acid cornmeal agar (CACA).The brown-colored colonies were recultured to observe their morphology,growth on canavanine-glycine-bromothymol-blue (CGB) medium,phenol oxidase and urease activities,serotype and mating type.There were obvious differences in the positive sample rates of C.neoformans in pigeon droppings collected from the different cities,ranging from 50％ in the cities located at latitudes from 30°N-40°N,29％ at 20°N-30°N and 13％ at 40°N-50°N.Conclusions There were no differences in positive bevy rates (approximately 80%) among the three grouped cities.Mycological tests of 101 isolates purified from pigeon droppings revealed that they were C.neoformans var.grubii.We also observed variable capsular size around the C.neoformans cells in colonies with variable melanin production and the bio-adhesion of the natural C.neoformans cells with other microorganisms.One urease-negative C.neoformans isolatewas isolated from pigeon droppings in Jinan city.No C.gattiiwas isolated in this study.

关键词

Cryptococcus neoformans/Cryptococcus gattii/ecology

Key words

Cryptococcus neoformans/Cryptococcus gattii/ecology

引用本文复制引用

LI An-sheng,CHEN Min,SHI Wei-ming,LIAO Wan-qing,PAN Wei-hua,WU Shao-xi,Taguchi Hideaki,GUO Ning-ru,SHEN Yong-nian,LU Gui-xia,PAN Ru-gui,ZHU Miao-chang..Ecological surveys of the Cryptococcus species complex in China[J].中华医学杂志（英文版）,2012,125(3):511-516,6.

基金项目

This study was supported by grants from the National Natural Science Foundation of China (No.80171335,No.30970130),the PLA Clinical High-tech Major Projects Fund (No.2010gxjs063),and Shanghai Science and Technology Commission Fund (No.10dz2220100). （No.80171335,No.30970130）

中华医学杂志（英文版）

OACSCDCSTPCDMEDLINESCI

ISSN：0366-6999

访问量3

下载量0

段落导航