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E-cadherin mediates adhesion and endocytosis of Aspergillus fumigatus blastospores in human epithelial cells

XU Xiao-yong SHI Yi ZHANG Peng-peng ZHANG Feng SHEN Yu-ying SU Xin ZHAO Bei-lei

中华医学杂志（英文版）2012，Vol.125Issue(4)：617-621,5.

中华医学杂志（英文版）2012，Vol.125Issue(4)：617-621,5.DOI:10.3760/cma.j.issn.0366-6999.2012.04.011

E-cadherin mediates adhesion and endocytosis of Aspergillus fumigatus blastospores in human epithelial cells

XU Xiao-yong ¹SHI Yi ¹ZHANG Peng-peng ¹ZHANG Feng ¹SHEN Yu-ying ¹SU Xin ¹ZHAO Bei-lei¹

作者信息

1. Department of Respiratory Medicine, Second Military Medical University/Nanjing General Hospital of Nanjing Military Command, People's Liberation Army, Nanjing, Jiangsu 210002,China
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摘要

Abstract

Background Aspergillus fumigatus (A.fumigatus) is a ubiquitous saprophytic fungus responsible for the majority of invasive mold infections in patients undergoing chemotherapy,organ transplantation or with persistent neutropenia.This study aimed to determine the role of E-cadherin for adhesion and endocytosis of A.fumigatus blastospores in the human epithelial cell line A549.Methods A.fumigatus blastospores were incubated with the total protein of A549 to investigate the binding of E-cadherin and blastospores followed by an affinity purification procedure.After establishing the adhesion model,the adhesion and endocytosis of A.fumigatus blastospores by A549 cells were evaluated by down-regulating E-cadherin of A549 cells using blocking antibody or small interfering RNA (siRNA).Results E-cadherin was adhered to the surface of A.fumigatus blastospore.Adhesion and endocytosis of the blastospores were reduced by blocking or down-regulating E-cadherin in A549 cells.Conclusions E-cadherin is a receptor for adhesion and endocytosis of A.fumigatus blastospores in epithelial cells.This may open a new approach to treat this fungal infection.

关键词

E-cadherin/receptor/A549/endocytosis/Aspergillus fumigatus

Key words

E-cadherin/receptor/A549/endocytosis/Aspergillus fumigatus

引用本文复制引用

XU Xiao-yong,SHI Yi,ZHANG Peng-peng,ZHANG Feng,SHEN Yu-ying,SU Xin,ZHAO Bei-lei..E-cadherin mediates adhesion and endocytosis of Aspergillus fumigatus blastospores in human epithelial cells[J].中华医学杂志（英文版）,2012,125(4):617-621,5.

基金项目

This work was supported by a grant from the National Natural Science Foundation of China (No.81000003). （No.81000003）

中华医学杂志（英文版）

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ISSN：0366-6999

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