首页|期刊导航|中华医学杂志（英文版）|Three-dimensional cultures of human endometrial cells on Matrigel mimic in vivo morphology
中华医学杂志（英文版）2012，Vol.125Issue(5)：863-868,6.DOI:10.3760/cma.j.issn.0366-6999.2012.05.024
Three-dimensional cultures of human endometrial cells on Matrigel mimic in vivo morphology
Three-dimensional cultures of human endometrial cells on Matrigel mimic in vivo morphology
摘要
Abstract
Background The regulation of endometrial physiology and morphogenesis by the paracrine effectors has been well established using in vivo studies.A more complete understanding of the endometrial function has been delayed due,in part,to a lack of appropriate culture models.In this study,we aimed to simulate the in vivo three-dimensional (3-D) growth pattern of endometrial cells using a 3-D in vitro culture system.Methods Isolated endometrial epithelial cells,stromal cells and RL95-2 cells were seeded into culture chambers coated with the extracellular matrix Matrigel and observed using light microscopy.Fluorescence staining and immunohistochemistry were used to assess the morphology.Results Depending on the culture conditions,epithelial cells and RL95-2 cells formed multicellular structures on Matrigel; stromal cells remained individually distinguishable or grew together to form 3-D lattice-like structures.Conclusions Matrigel provided a good microenvironment for culturing endometrial cells.The cells cultured in the Matrigel-coated chambers closely resembled those seen in vivo.关键词
Matrigel/endometrium/three-dimensional culture/morphologyKey words
Matrigel/endometrium/three-dimensional culture/morphology引用本文复制引用
ZHU Hai-yan,WANG Jun-xia,TONG Xiao-mei,XU Wei-hai,JIANG Ling-ying,JING Xiao-ying,YANG Ling-yun,ZHOU Feng,ZHANG Song-ying..Three-dimensional cultures of human endometrial cells on Matrigel mimic in vivo morphology[J].中华医学杂志（英文版）,2012,125(5):863-868,6.基金项目
The research was supported by the grants from Department of Education of Zhejiang (No.Y200909794) and from the Natural Science Program of Zhejiang (No.Y2100075). （No.Y200909794）
