中华临床免疫和变态反应杂志2011，Vol.5Issue(4)：253-257,5.
蟑螂主要过敏原Blag7单克隆抗体的制备与鉴定
Production and Identification of Monoclonal Antibodies Against Cockroach Allergen Bla g7
摘要
Abstract
Objective Anti-cockroach allergen ( Bla g7) monoclonal antibodies (mAb) were produced and identified. Methods The Bla g7 was expressed in Origami/pGS2la E. Coli strain, and highly purified by affinity chromatography. BALB/c mice were immunized with Bla g7 protein and hybridoma cell lines were obtained by limited dilution procedure. The specificity of mAbs was characterized by enzyme-linked immunosorbent assay ( ELISA) and Western blot. Results Two hybridoma cell lines that secreted mAbs against Bla g7 were obtained, named Bla g7-l and Bla g7-2. The subtype of the two mAbs belongs to the IgGl. The titers of mAbs in the culture supernatant were 1: 16 000 and 1: 8 000 respectively, which were detected by indirect ELISA. Bla g7-l and Bla g7-2 mAbs can specifically bind with Bla g7, shown by Western blotting. Conclusion Bla g7 was successfully expressed and purified. Two mAbs against Bla g7 were obtained which will provide valuable tools for studies on cockroach related allergic diseases.关键词
过敏原,蟑螂(Bla g7)/单克隆抗体/免疫印迹Key words
cockroach allergen (Bla g7)/ monoclonal antibodies/ Western blot分类
医药卫生引用本文复制引用
马文静,张伟,魏继福,张浩,刘成成,何韶衡..蟑螂主要过敏原Blag7单克隆抗体的制备与鉴定[J].中华临床免疫和变态反应杂志,2011,5(4):253-257,5.基金项目
国家自然科学基金(30972714),江苏省自然科学基金攀登项目(BK2010020) （30972714）
