中华临床免疫和变态反应杂志2011，Vol.5Issue(4)：272-277,6.
环磷酰胺在野百合碱诱导大鼠肺动脉高压模型中的作用
Effectiveness of Cyclophosphomide in Monocrotaline-induced Pulmonary Arterial Hypertension Model
摘要
Abstract
Objective To evaluate the efficacy of cyclophosphamide ( CTX) in monocrotaline (MCT) -induced pulmonary arterial hypertension ( PAH) model and to investigate the possible mechanism. Methods Rats received MCT (60 mg/kg intraperitoneal injection, I. P. ) once, CTX treatment groups were divided into prophylactic and remedial intervention. At the end of week 2 and week 4, compare the mean pulmonary arterial pressure (mPAP) and "R value" of the prophylactic treatment group or remedial treatment group with of 2-week model group or 4-week model group respectively; immunohistochemistry staining was used to determine the expression of tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) in the rat lung tissues. Results Remedial CTX intervention can significantly reduce the mPAP ( compared with the 4-week model group, P <0. 01), but prophylactic group showed no tendency of mPAP-reduction. Using R value toassess the efficacy of the remedial group, we found the same results. The immunohistochemistry staining showed there were massive expressions of TNF-α and IL-6 in the lung tissues of model groups and decreased, staining in remedial groups. Conclusions Inhibition of TNF-a and IL-6 with CTX in MCT-induced PAH model was resulted in a significant reduction of mPAP and thickness of small pulmonary arteries. Inflammation may play a key role in the pathogenesis of MCT-induced PAH model. CTX may be useful in the therapeutic intervention of connective tissue disease associated with PAH.关键词
肺动脉高压/野百合碱/环磷酰胺/炎症因子Key words
pulmonary arterial hypertension/ monocrotaline/ cyclophosphomide/ inflammatory factors分类
医药卫生引用本文复制引用
卢杰,李梦涛,王迁,何建国,曾小峰..环磷酰胺在野百合碱诱导大鼠肺动脉高压模型中的作用[J].中华临床免疫和变态反应杂志,2011,5(4):272-277,6.基金项目
国家"十一五"科技支撑计划课题(2006BAI01 A07和2008BA159B02),中华医学会临床医学科研专项资金项目(08010270105) （2006BAI01 A07和2008BA159B02）
