浙江大学学报（理学版）2011，Vol.38Issue(6)：689-694,6.DOI:10.3785/j.issn.1008-9497.2011.06.016
山荷叶离体培养和种质试管保存技术研究
Study on techniques of tissue culture and germplasm conservation in vitro of Astilbe tabularis (Hemsl.) Engler
摘要
Abstract
The tender leaves of Astilbe tabularis (Hemsl. ) Engler were used as explants, all suitable media of tissue culture and germplasm conservation in vitro were screened through uniform design experiments. The results showed that MS+6-BA1. 00 mg·L-1 + IBA0. 08 mg·L~' was most suitable for callus induction with a induction rate of 99. 7% ; MS+6-BA2. 80 mg·L-1 +IBA0. 02 mg·L-1 +GA30. 90 mg·L-1 was most suitable for shoots regeneration with a regeneration rate of 99. 5%; 1/6MS+IAA0. 02 mg·L-1 +IBA0. 04 mg·L-1 was most suitable for rooting with a rooting rate of 99. 2%; 1/4MS+6-BA0. 10 mg·L-1 +phloridzin 2. 50 mg·L-1 was most suitable for germplasm conservation in vitro for 27 months with a growing rate of 7. 9%. The results proved that tissue culture system of Astilbe tabularis (Hemsl. ) Engler has been successfully established, and the method of defering growth was utilized for germplasm conservation in vitro at normal temperature.关键词
山荷叶/种质试管保存/均匀设计/根皮苷Key words
Astilbe tabularis (Hemsl. ) Engler/ germplasm conservation in vitro/ uniform design/ phloridzin分类
农业科技引用本文复制引用
顾地周,陆爽,朱俊义,陈霞..山荷叶离体培养和种质试管保存技术研究[J].浙江大学学报（理学版）,2011,38(6):689-694,6.基金项目
国家科技部"国家科技攻关计划引导项目"(2005BA741C). （2005BA741C）
