浙江农林大学学报2011，Vol.28Issue(6)：911-917,7.
碳源对山核桃体细胞胚发生和植株再生的影响
Somatic embryogenesis and plant regeneration from Carya cathayensis embryos using different carbon sources
摘要
Abstract
A process for studying somatic embryogenesis and plant regeneration in vitro from immature embryos of Carya cathayensis, which were 10-12 weeks post-pollination, was developed. To select a carbon source (glucose, sucrose, trehalose, and maltose) for embryonic callus, a single-factor experimental design was used. Then, the optimal concentration of glucose (10,20,40,60 and 80 g·L-1) was determined. Also, for somatic embryogenesis, the best carbon source was decided. Then, the optimal sucrose concentrations (15, 30, 45, 60 and 75 g·L-1) for somatic embryogenesis and for somatic embryo germination were determined. Results showed that the embryogenic callus induction rate of immature embryos inoculated in the medium supplemented with glucose was significantly higher (P < 0.05) than sucrose, trehalose, and maltose. Embryogenic callus percentage was significantly greater (P < 0.05) with 20 g·L-1 glucose with a 50.0%. For somatic embryogenesis of immature embryos, sucrose as a carbon source was significantly better (P < 0.05) than glucose, trehalose, and maltose. Somatic embryogenesis induction was significantly higher (P < 0.05) with 45 g-L-1 sucrose. For somatic embryo germination, a basal woody plant medium (WPM) supplemented with 5 g·L-1sucrose was significantly higher (P < 0.05) than other concentrations. [Ch, 6 fig. 26 ref.]关键词
经济林学/山核桃/碳源/体细胞胚/植株再生Key words
cash forestry/ Carya cathayensis/ carbon source/ somatic embryogenesis/ plant regeneration分类
农业科技引用本文复制引用
胡恒康,江香梅,张启香,陈贝,黄坚钦..碳源对山核桃体细胞胚发生和植株再生的影响[J].浙江农林大学学报,2011,28(6):911-917,7.基金项目
浙江省自然科学基金重大项目(Z307534) （Z307534）
浙江省自然科学基金资助项目(Y3090091) （Y3090091）
浙江省科学技术攻关项目(2009C32027) （2009C32027）
浙江农林大学B类团队创新项目(2007) （2007）
浙江省大学生科技创新项目(2008) （2008）
