肿瘤防治研究2011，Vol.38Issue(12)：1346-1350,5.DOI:10.3971/j.issn.1000-8578.2011.12.002
CD44+鼻咽癌细胞的干细胞生物学特性
Stem Cell Biological Characteristics of CD44+ in Human Nasopharyngeal Carcinoma
摘要
Abstract
Objective To separate, expansion, and explore the biological feature of CD44+ cells from the human nasopharyngeal carcinoma (NPC) SUNE-1 5-8F cell line. Methods Flow cytometry was used to detect the expression of CD44 in SUNE-1 5-8F. Fluorescence-activated Cell Sorting was applied to purify CD44+ cells. MTT assay or clone formation assay was used to detect the differences of CD44+ and CD44" cells in proliferation and differentiation ability. The expression of stem cell markers C)ct4 and Bmi-1 was examined by reverse transcriptase polymerase chain reaction (RT-PCR). Results CD44 was positively expressed in about 52. 5% of NPC SUNE-1 5-8F cell line. Regardless of serum-free medium and serum medium culture conditions, freshly sorted CD44+ cells showed stronger proliferative capacity than CD44- and unsorted CD44+ cells. The expression levels of Bmi-1 and Oct4 mRNA in CD44+ cells were significantly higher than that in CD44" cells. After 2Gy radiation, the average clone formation efficiency for CD44 + and CD44- cells was(23. 44± 1. 90)% and (7.78±1. 17) %( P<0. 001), respectively. After Cisplatin and Docetaxel treatment, CD44+ cells showed a higher survival rate in the same drug concentration compared with CD44- cells. Conclusion CD44+ cells have the biological characteristics of tumor stem cell and may be assumed as one of markers of NPC tumor stem cells.关键词
鼻咽肿瘤/肿瘤干细胞/CD44/生物学特性Key words
Nasopharyngeal Carcinoma/ Tumor stem cell/ CD44/ Bionomics分类
医药卫生引用本文复制引用
许新华,苏进,鲁明骞,李道俊,黄乔,薛峰,易芳..CD44+鼻咽癌细胞的干细胞生物学特性[J].肿瘤防治研究,2011,38(12):1346-1350,5.基金项目
湖北省卫生厅科研资助项目(JX4B52) （JX4B52）
2011年宜昌市科技研究与开发项目(A11301-04) （A11301-04）
宜昌市中心人民医院2008年科研发展基金项目(KFJ2008005) （KFJ2008005）
