肿瘤防治研究2011，Vol.38Issue(12)：1351-1355,5.DOI:10.3971/j.issn.1000-8578.2011.12.003
腺病毒介导的shRNA沉默hTERT基因表达对鼻咽癌细胞增殖和凋亡的影响
Effect of Adv Vector-mediated shRNA Targeting hTERT on Proliferation and Apoptosis of Nasopharyngeal Carcinoma Cells
摘要
Abstract
Objective To evaluate the effect of targeting hTERT gene on the proliferation and apoptosis of CNE-2 cells by applying RNA interference to restrain the expression of hTERT in nasopharyngeal carcinoma CNE-2 cells. Methods Recombinant adenovirus vectors expressing EGFP and human TERT shR-NA were construted and transfected in human nasopharyngeal carcinoma CNE-2 cells. The expression levels of hTERT mRNA and protein were detected respectively by RT-PCR and Western blot method. Cell proliferation was determined by CCK-8 assay and cell apoptosis was observed by PCM (Flow cyto-metric). Results The transfection rate of Adv-EGFP-shTERT recombinant adenovirus plasmid in CNE-2 cell line was more than 90 %. The expression levels of the hTERT mRNA and protein were dramatic declining respectively at 24h and 48h after transfection. Cell proliferation activity was significantly inhibited and the cell apoptosis reached 23. 0%. Conclusion Adv vector-mediated RNAi targeting hTERT can inhibit the activity of telomerase reverse transcriptase by down-regulating the expression of the hTERT mRNA and its protein significantly, therefore can inhibit the growth of the cells and induce their apoptosis. Future application of RNAi to the gene therapy of nasopharyngeal carcinoma might be expected.关键词
RNA干扰/腺病毒载体/hTERT基因/NPC细胞Key words
RNA interference/ Viral vectors/ hTERT genes/ NPC cells分类
医药卫生引用本文复制引用
宋玉姣,韩继波,陈始明,肖伯奎,陈晨,陶泽璋..腺病毒介导的shRNA沉默hTERT基因表达对鼻咽癌细胞增殖和凋亡的影响[J].肿瘤防治研究,2011,38(12):1351-1355,5.基金项目
国家自然科学基金资助项目(30872851) （30872851）
