肿瘤防治研究2011，Vol.38Issue(12)：1367-1369,3.DOI:10.3971/j.issn.1000-8578.2011.12.007
雷帕霉素上调Beclin1诱导肝癌细胞死亡的体外研究
Rapamycin Induces Cell Death of Hepatoma Cells via Upregulation of Beclin 1
摘要
Abstract
Objective To investigate the rapamycin-induced cell death and the time and dose-effect relationship. Methods Cell death ratio was analyzed by using trypan blue exclusion assay. The rapamycin-induced cell death fashion was detected by TEM in SMMC7721. Furthermore, Beclin 1 was detected by using Western blot Results Rapamycin significantly induces liver cell death, and has significant time and dose-effect dependence, Ra-pamycin can induce both apoptosis and autophagy in SMMC7721. Beclin 1 was upregulated by rapamycin. Con-duskn Rapamycin can induce apoptosis and autophagic cell death in hepatoma cells, Beclin 1 upregulation may be the direct cause for rapamycin-induced cell death in HGC cells.关键词
肝癌/雷帕霉素/凋亡/自噬性细胞死亡/Beclin 1Key words
Hepatocellular carcinoma/ Rapamycim Apoptosis /Autophagic cell death/Beclin 1分类
医药卫生引用本文复制引用
杨莹,冯浩,景晓红,苟兴春..雷帕霉素上调Beclin1诱导肝癌细胞死亡的体外研究[J].肿瘤防治研究,2011,38(12):1367-1369,3.基金项目
陕西省教育厅科研计划资助项目(2010JK801,2010JK804) （2010JK801,2010JK804）
陕西省卫生厅卫生科研基金资助项目(2010H26) （2010H26）
