肿瘤防治研究2012，Vol.39Issue(1)：6-8,3.DOI:10.3971/j.issn.1000-8578.2012.01.002
热休克预处理对高温致中国仓鼠肺细胞(V79)细胞DNA双链断裂的影响
Influences of Heat Shock Pretreatment on DNA Double-strand Breaks Damaged by High Temperature of V79 Cells
摘要
Abstract
Objective To study the influences of different heat shock pretreatment temperature on DNA double-strand breaks damaged by high temperature of V79 cells. The DNA double-strand breaks foci were acquired on a accurate laser-scanning confocal microscope and y-H2AXs were incubated with anti-γ-H2AX antibodies. Methods V79 cells was cultured and divided into control group and groups with 39℃, 40℃ ,41℃ ,42℃ heat shock pretreatment. After heat shock pretreatment and recover for 6 hours, cells were damaged with high temperature of 43℃ and 45℃. Fixed with 4% paraformaldehyde,cells were incubated with anti-γ-H2AX antibodies overnight and subsequently labelled with goat-anti-mouse IgG conjugated with FITC for 1 hour. The DNA double-strand breaks foci were acquired on a laser-scanning confocal microscope. Results Foci images were acquired on a laser-scanning confocal microscope. DNA double-strand breaks foci in control group and groups with heat shock pretreatment have significant differences (P<0. 05); Conclusion Heat shock pretreatment can decrease foci of DNA double-strand breaks induced by high temperature treatment. V79 cells can present adaptability to high temperature.关键词
热休克预处理/γ-H2AX/激光共聚焦显微镜/DNA双链断裂Key words
Heat shock pretreatment/γ-H2AX/Laser-scanning confocal microscope (LSCM) /DNA double-strand breaks(DSBs)分类
医药卫生引用本文复制引用
李明,张保国..热休克预处理对高温致中国仓鼠肺细胞(V79)细胞DNA双链断裂的影响[J].肿瘤防治研究,2012,39(1):6-8,3.基金项目
国家自然科学基金资助项目(10275083) （10275083）
