肿瘤防治研究2012，Vol.39Issue(1)：9-12,4.DOI:10.3971/j.issn.1000-8578.2012.01.003
5-氮杂-2'-脱氧胞苷对肝癌细胞HepG2凋亡及其PEG10基因表达的影响
Effects of 5-Aza-2'-deoxycytidine on Apoptosis and Expression of PEG10 Gene in Human Hepato Carcinoma Cell Line HepG2
摘要
Abstract
Objective To investigate the effects of demethyhfing agent 5'-Aza-2'-deoxyeytidine(5-Aza-CdR)on the expression of PEG10 gene in human hepato carcinoma cell line HepG2. Methods HepG2 cells were treated with 50. 0 μmol/L 5-Aza-CdR for 24,48,72 h. Apoptosis of HepG2 induced by 5-Aza-CdR was identified by fluorescence microscopy with hoechst 33342/PI staining. RT-PCR and Western blot were applied to detect the PEGU) mRNA and protein expression levels, respectively. Results After treated with 5-Aza-CdRat for indicated time,fluorescence microscopy analysis showed that the apoptosis rate of HepG2 cells increased significantly, and the expression of PEG10 mRNA and protein in HepG2 cells were reduced in a time-dependent manner(P<0. 05). Conclusion 5-Aza-CdR can induce HepG2 cell apoptosis and reduce the expression of PEG10 gene.关键词
5-氮杂-2'-脱氧胞苷/PEG10/凋亡/HepG2细胞Key words
5-Aza-CdR/PEG101 Apoptosis /HepG2 cell分类
医药卫生引用本文复制引用
刘瑶,贺兴波,谢军,孟凡,杨建琼,黄才斌..5-氮杂-2'-脱氧胞苷对肝癌细胞HepG2凋亡及其PEG10基因表达的影响[J].肿瘤防治研究,2012,39(1):9-12,4.基金项目
国家自然科学基金资助项目(81000908) （81000908）
江西省教育厅科技项目(GJJ11210) （GJJ11210）
