肿瘤防治研究2012，Vol.39Issue(1)：13-17,5.DOI:10.3971/j.issn.1000-8578.2012.01.004
TIP30基因对大肠癌细胞HCT116生物学特性的影响
Effect of TIP30 on Biological Characters of HCT116 Cells
摘要
Abstract
Objective To study the association of TIP30 and biological characters of HCT116 colorectal cancer cells. Methods The eukaryotic expression vector of TIP30 gene was established and transfected into HCT116 cells by liposome-mediated transfection. The expressions of TIP30 gene mRNA and protein were detected by RT-PCR and Western blot. The invasive ability was analyzed by Matrigel invasion assay. The colony formation assay was used to detect cell growth inhibition. Results The stable transfec-tant of HCT116/pCMV4-flag-TIP3() cell was established successfully, the proliferation of HCT116/pC-MV4-flag-TIP30 cell was inhibited,the invasive ability and colony formation ability were also decreased compared to control cell. Conclusion The overexpression of TIP30 gene in HCT116 cells not only results in inhibiting cell growth and cell apoptosis.but also reducing cell migration and invasion, which provides evidence for TIP30 gene therapy.关键词
TIP30基因/DNA甲基化/大肠癌/抑癌基因Key words
TIP30 gene/DNA methylation/Colorectal cancer/Tumor suppressor gene分类
医药卫生引用本文复制引用
吕慧芳,刘红亮,陈小兵,陈贝贝,李宁,邓文英,马磊,罗素霞..TIP30基因对大肠癌细胞HCT116生物学特性的影响[J].肿瘤防治研究,2012,39(1):13-17,5.基金项目
河南省医药卫生重大攻关项目及卫生部科研基金资助项目(WKJ2007-2-026) （WKJ2007-2-026）
