肿瘤防治研究2012，Vol.39Issue(1)：71-74,4.DOI:10.3971/j.issn.1000-8578.2012.01.018
BAG-1在大肠癌中的表达及其临床意义
Expression and Clinical Significance of BAG-1 in Human Colorectal Carcinomas
杨光华 1赵晶 1李磊 1王天阳 1张小艳 1吕春秀 2王凤安1
作者信息
- 1. 050000 石家庄,河北医科大学第二医院胃肠外科
- 2. 050000 石家庄,河北医科大学第四医院肿瘤内科
- 折叠
摘要
Abstract
Objective To investigate the relationship and clinical significance of Bag-1 expression in colorectal carcinoma tissues and normal colorectal tissues, and its relationship with lymphnode metastasis. Methods The expressions of Bag-1 in 55 patients' colorectal carcinoma tissues (case group) and 25 normal colorectal tissues (control group) were examined by Western blot analysis and RT-PCR. Results (1) The expressions of Bag-1 in 55 colorectal carcinoma tissues were inordinately detected, the protein relative strength of Bag-1 was (5~109,64. 09 ± 31. 59). We found that the protein relative strength of Bag-1 in colorectal carcinoma tissues with lymphnode metastasis (30. 34~109,81. 05 ± 19. 64) was higher than that in colorectal carcinoma tissues without lymphnode metastasis (5~70. 13,26. 19 ± 16. 32) (P<0. 001),however,both of them were obviously higher than that in normal colorectal tissues (0~10,4. 28 ± 2. 82)(P<0. 001). (2) The gene fragments of Bag-1 and β-actin primers amplified were clearly visible by electrophoresis and EB staining. The length of the gene fragments amplified was 331 bp and 254 bp respectively, which was consistent with that we designed. The relative expression value of Bag-1 mRNA in 55 colorectal carcinoma tissues was (0. 33- 1. 21,0. 74 ± 0. 26). The relative expression value of Bag-1 mRNA in colorectal carcinomas tissues with lymphnode metastasis (0. 52~ 1. 21,0. 87 ± 0. 21) was higher than that in colorectal carcinoma tissues without lymphnode metastasis (0. 33-0. 65,0. 45 ±0. ()9)(P<0. 001), however, both of them were significantly higher than that in normal colorectal tissues(0~0. 22,0. 10 ± 0. 06)(P<0. 001). Conclusion The overexpression of Bag-1 plays an important role in the development and progression of colorectal carcinomas. The expression level of Bag-1 is correlated with lymphnode metastasis. Bag-1 can serve as a biomarker to prognosticate the metastasis potentiality of colorectal carcinomas. Detecting Bag-1 may contribute to estimate the prognosis of patients.关键词
Bag-1/大肠癌/RT-PCR/Western blotKey words
Bag-1/Colorectal carcinoma/RT-PCR/Western blot分类
医药卫生引用本文复制引用
杨光华,赵晶,李磊,王天阳,张小艳,吕春秀,王凤安..BAG-1在大肠癌中的表达及其临床意义[J].肿瘤防治研究,2012,39(1):71-74,4.
