肿瘤防治研究2012，Vol.39Issue(1)：75-77,3.DOI:10.3971/j.issn.1000-8578.2012.01.019
结肠癌组织和癌旁组织miRNA表达谱研究
microRNA Expression Profile between Colon Cancer Tissues and Noncancerous Tissues Surrounding Colon Cancer
谢海涛 1庄俊华 2黄宪章3
作者信息
- 1. 510006 广州,广州中医药大学
- 2. 南华大学附属第一医院检验科
- 3. 广州中医药大学第二附属医院检验科
- 折叠
摘要
Abstract
Objective To profile the miRNA expression pattern between colon cancer tissues and noncan-cerous tissues surrounding colon cancer. Methods The expression level of miRNAs in tumor and adjacent normal tissue from clinical colon cancer patients without metastasis was detected by Chip-array. The expression level of miRNA was measured by Real-time PCR. Results Down-regulation of 24 miRNAs and up-regulation of 27 miRNAs were identified in tumor tissue compared with adjacent normal tissue. The expression level of miR-145 and miR-143 was significantly down-regulated in tumor tissue, while the expression level of miR-451 was increased in tumor tissue. All results were validated by Real-time PCR. Conclusion The expression profile of miRNA is different between tumor tissue and adjacent normal tissue. Expression level of miR-145 and miR-143 is significantly down-regulated in colorectal tumor, while expression level of miR-451 is increased.关键词
结肠癌/miR-145/miRNAKey words
Colon cancer/miR-145/microRNA分类
医药卫生引用本文复制引用
谢海涛,庄俊华,黄宪章..结肠癌组织和癌旁组织miRNA表达谱研究[J].肿瘤防治研究,2012,39(1):75-77,3.
