中南民族大学学报：自然科学版2011，Vol.30Issue(3)：45-48,4.
中药复方板蓝根颗粒抗柯萨奇B_4病毒作用的实验研究
Antiviral Activity of the Prescription from Radix Isatidis Against Coxackievirus B_4 Virus in Vitro
摘要
Abstract
To determine the antiviral activity of the traditional Chinese medicine,the prescription from Radix Isatidis,against Coxackievirus B4（CVB4） virus,cell culture technigue to observe cytopathic effect（CPE） and MTT colorimetric assay were used to obtain the virus inhibitory rate.The results showed that the prescription from Radix Isatidis may inhibit the CVB4 biological synthesis and block its adsorption to Hep-2 cells rather than inactivate this virus directly.The 50% inhibitory concentrations（IC50） of the above 2 techniques were 1129.00 μg/mL and 1130.44 μg/mL respectively,and their treatment indexes（TI） were both relatively found to be 2.78.Moreover,there was obvious dosage-effect relationship between the 100~1600 μg/mL dose of the prescription and its anti-CVB4 inhibitory rate in vitro（P0.01）.1600 μg/mL of the prescription from Radix Isatidis can inhibit more than 50% of the proliferation of CVB4 in Hep-2 cells.It is conclude that the prescription from Radix Isatidis can inhibit the adsorption and profication of the CVB4 in Hep-2 cell effectively.关键词
复方板蓝根颗粒/柯萨奇病毒B4/抗病毒作用Key words
Radix Isatidis/CVB4/antiviral activity分类
医药卫生引用本文复制引用
刘钊,赵鹏,杨占秋..中药复方板蓝根颗粒抗柯萨奇B_4病毒作用的实验研究[J].中南民族大学学报：自然科学版,2011,30(3):45-48,4.基金项目
中南民族大学自然科学基金资助项目(YZQ05011) （YZQ05011）
