中山大学学报（医学科学版）2012，Vol.33Issue(1)：8-15,8.
Combretastatin A4 Phosphate对人晶状体上皮细胞增殖及迁移的影响
Effect of Combretastatin A4 Phosphate on Proliferation and Migration of Lens Epithelial Cells
摘要
Abstract
[Objective]To study the effect of combretastatin A4 phosphate (CA4P) on proliferation and migration of lens epithelial cells line SRA01 / 04 and discuss the mechanism on CA4P inhibiting cell proliferation. [Methods] The experimental object was lens epithelial cells line SRA01/04. We divided them into the control group and CA4P group, treating respectively by PBS or lumol/ L CA4P for 15 min. The testing time were Oh, 24 h, 48 h, and 72 h after drug-treating. Cell proliferation capability was detected by WST -1. Cell migration was detected by wound test. Way of cell death was detected by Annexin V-FITC/PI. Cell cycle was detected by PI streaming. Cytoskeleton protein (alpha microtubules protein, F-actin) and cell morphology were detected by immunofluorescence. [Results] Inhibition of cell proliferation by CA4P presented time dependent. After 24 h 48 h, and 72 h treated by CA4P, the cell viability were 73%, 40%, and 34%. Wound test showed a large number of cells covers scratches in the control group. Scratches in CA4P group was smooth. Cells become tiny and round, falling off the basement to floating. Cell apoptosis in CA4P group were 19.7%, 43.6%, and 72.2% in 24 h, 48 h, and 72 h, respectively. It was conspicuously higher than those in the control group (1.7%, 3.6%, and 2.2%). G2/M period distribution in CA4P group were 30.3%,55.4%,and 70.8% in each test points respectively, which was significantly higher than those in the control group (4.4% 9.0%, and 7.9%). Microtubules fiber filamentous structure disappeared in CA4P group. Cells appeared mitotic obstacle performances, such as multi-core and huge nuclear. But F-actin showed no changes. [Conclusion] 1 μmol/L CA4P obviously inhibit the proliferation and migration of lens epithelial cells by short-term incubation. The effect is time dependent. The mechanism of CA4P inhibiting cell proliferation probably relate to depolymerizationmicrotubules protein which induce mitotic catastrophe and apoptosis.关键词
后发性白内障/晶状体上皮细胞/Combretastatin A4 Phosphate/微管蛋白Key words
posterior capsule opacification/ lens epithelial cell/ combretastatin A4 phosphate/ microtubules protein分类
医药卫生引用本文复制引用
王艳,朱丽媛,刘畅,刘良平,叶少碧,吴明星,刘奕志..Combretastatin A4 Phosphate对人晶状体上皮细胞增殖及迁移的影响[J].中山大学学报（医学科学版）,2012,33(1):8-15,8.基金项目
国家自然科学基金(81070719) （81070719）
卫生部临床学科重点项目(20100439) （20100439）
