中山大学学报（医学科学版）2012，Vol.33Issue(1)：39-43,5.
HBV-DNA高水平孕妇的HBV前S和S区基因突变及基因分型
Genetic Mutations and Genotypesof HBV Pre S and S Regions in Pregnant Women with High Level Serum HBV-DNA
摘要
Abstract
[Objective] To provide an experimental basis for exploring the reason of HBV maternal vertical transmission immune blocking failure, we studied the mutation profiles and genotypes of hepatitis B virus ( HBV) in the Pre S and S regions of pregnant women with high level serum HBV-DNA before delivery. [Methods] HBV DNA were extracted from the serum of 41 pregnant women with HBsAg positive and HBV-DNA ≥ 105 IU/mL. Based on the sequence alignment of all HBV genotypes, specific primers targeting all HBV genotypes were designed to amplify the Pre S and S regions of HBV followed by sequence analysis on the sequencing data available. [Results] 1. Among the 41 cases, 29 cases (70.73%) showed mutations in the Pre S and S regions, substitution was the main mutation model. Among all cases, PreSl, pre S2 and S regions mutations frequency were 8.96%, 9.88%, and 3.24%, respectively, mutation frequency in pre SI and pre S2 regions were significantly higher than that in S region (P < 0.05). 2. Genotypes based on the mutation in S region and homology tree model were analyzed. In the 41 cases, 22 cases were genotype B, and 19 cases were genotype C. The mutation frequency in the Pre S and S regions between genotype B and genotype C were not statistically significant (P > 0.05). Genotype B and genotype C had their own clusters, homology rate was less than 90% between cluster B and cluster C, but in the cluster B and cluster C the homology rate were 96% and 95%, respectively. [Conclusion] 1. Mutations of HBV Pre S and S regions were common in pregnant women with high level serum HBV-DNA before delivery. The most common mutation in the Pre S and S regions of HBV was substitution, mutation frequency in pre SI and pre S2 regions were higher than that in S region. 2.The homology rate between different genotypes was low, and study on the mutation pattern of HBV Pre S and S regions should be analyzed independently in each genotype.关键词
乙型肝炎病毒/HBV-DNA定量/前S和S基因区/基因突变/基因型Key words
Hepatitis B virus/ HBV-DNA level/ Pre S and S regions/ Genetic mutation/ genotype分类
医药卫生引用本文复制引用
尹玉竹,张培珍,章钧,周瑾,侯红瑛..HBV-DNA高水平孕妇的HBV前S和S区基因突变及基因分型[J].中山大学学报（医学科学版）,2012,33(1):39-43,5.基金项目
广东省科技计划项目(2008B060600023) （2008B060600023）
