中山大学学报（医学科学版）2012，Vol.33Issue(1)：111-115,5.
新型脱细胞脑组织修复支架的制备及其细胞相容性
Preparation and Cytocompatibility of Acellular Rat Brain Matrix for Tissue Engineering Scaffolds
摘要
Abstract
[Objective] To prepare the acellular rat brain matrix scaffold and detect its cytocompatibility with neural stem cells. [Methods] After anesthesia with 10% chloral hydrate, Sprague Dawley rats' brain was removed, and then cerebral cortex was isolated. Being made use of freeze-thaw, Triton X-100, sodium deoxycholate, DNA enzyme, and RNA enzyme, the acellular scaffold of rat brain tissue was prepared, and after being cross-linked with Genipin, an initial acellular rat brain matrix scaffold was made. Then, Haematoxylin and Eosin staining (HE) was used to evaluate its content. Scanning elextron microscopy (SEM) was used to view the ultrastructure of the samples and measure the pore size of specimens. Immunohistochemical analysis of acellular scaffold was carried out to seek the distribution and content of the three major extracellular matrix-FN, LN and Col IV. The cytocompatibility of the acellular scaffold was assessed by observing NSCs' growth, proliferation, and adhesion when co-cultured with the scaffold. [Results] After being treated by a variety of reagents, cells within rat brain tissue had been largely removed. The acellular scaffold had forms of three-dimensional network structure, good hydrophilicity, and a high degree of bionic. After being cross-linked by Genipin, the three-dimensional scaffold had become more porous, with an average pore size (14.9±2.5) μm , porosity was (90.4±2.2)%. The degradation of scaffold, and no significant residual cell debris within scaffold was found. LN expression in the acellular scaffold was strong positive, while FN and Col IV expression were weakly positive. Had been cultured onto cross-linked scaffolds for 7 days, the NSCs had a pretty proliferation and adhesion to the scaffold. HE staining showed that there were a large number of cells in extracellular matrix. SEM showed that a large number of NSCs adhere to the surface of material, and some cells grew processes. [Conclusion] The initial development of the acellular rat brain matrix scaffold has the structure of three-dimensional network, partly retains the extracellular matrix and has good cytocompatibility.关键词
脱细胞脑组织/支架/交联Key words
acellular rat brain matrix/ scaffolds/ cross-linking分类
医药卫生引用本文复制引用
何海勇,李文胜,王辉,蔡梅钦,龚瑾,郭英..新型脱细胞脑组织修复支架的制备及其细胞相容性[J].中山大学学报（医学科学版）,2012,33(1):111-115,5.基金项目
国家自然科学基金(30672152) （30672152）
广东省科技计划项目(2007B03100302) （2007B03100302）
