中兽医医药杂志2011，Vol.13Issue(5)：14-17,4.
兰州大尾羊mtDNA D-loop和Cytb区序列分析与多态性研究
Sequence and polymorphism analysis of mtDNA D-loop and Cytb region of Lanzhou fat tailed sheep
摘要
Abstract
The genetic polymorphisms of 20 Lanzhou fat tailed sheep were analyzed mainly by mtDNA sequence analysis.The results showed that the length of D-loop in mtDNA was 1 210 bp,and the content of base A,T,G,C,A＋T and G＋C was 29.0%,32.3%,23.5%,15.2%,61.3% and 38.7%,respectively.Mutation analysis and homology comparison showed that the nucleotide diversity was 0.037 85,thus 7 Lanzhou fat tailed sheep were from 3 different mothers with a near genetic distance.While the length of Cytb in mtDNA was 1 580 bp,and the content of base A,T,G,C,A＋T and G＋C was 27.2%,33.7%,26.7%,12.4%,60.9% and 39.1%,respectively.Software DNAsp4.10 analysis showed there were 14 haplotypes in the sequence of Cytb in mtDNA of 17 Lanzhou fat tailed sheep,in which number 1 and number 10,number 2 and number 5 shared one haplotype,respectively.These indicated that the ratio of haplotype was high in Lanzhou fat tailed sheep with low genetic polymorphism.关键词
兰州大尾羊/线粒体DNA/D-loop/线粒体DNA/Cytb/多态性Key words
Lanzhou fat tailed sheep/mtDNA D-loop/mtDNA Cytb/polymorphism分类
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李栋元,李景云,杨具田,臧荣鑫,李贞子,严建鹏,武春丽..兰州大尾羊mtDNA D-loop和Cytb区序列分析与多态性研究[J].中兽医医药杂志,2011,13(5):14-17,4.基金项目
甘肃省科技支撑计划 （）
