中兽医医药杂志2011，Vol.13Issue(6)：36-38,3.
酸性染料比色法测定红茂草中总生物碱含量
Determination of total alkaloid in Dicranostigma leptodum（Maxim.） Fedde by acid dye colorimetry
摘要
Abstract
The content of total alkaloid in Dicranostigma leptodum（Maxim.） Fedde（DLF） was determined by acid dye colorimetry.Using the isocorydine as standard,acid dye colorimetry conditions were as follows： pH=4.0,wavelength of 425 nm,bromocresol green liquid 1.5 mL.The standard curve was then obtained： y=0.032 x-0.033,R2=0.977 0.The concentration of alkaloid showed good linearity with its absorbability within the range of 21.6-54.0 mg/mL.The content of total alkaloid from DLF was 1.386%.The method is simple,highly sensitive and reproducible.关键词
酸性染料比色法/红茂草/总生物碱Key words
acid dye colorimetry/Dicranostigma leptodum（Maxim.） Fedde/total alkaloid分类
畜牧业引用本文复制引用
赵强,王廷璞,董晓宁,白斌峰,赵海福..酸性染料比色法测定红茂草中总生物碱含量[J].中兽医医药杂志,2011,13(6):36-38,3.基金项目
甘肃省中医药管理局基金资助项目 （）
