作物学报2011，Vol.37Issue(11)：1967-1974,8.DOI:10.3724/SP.J.1006.2011.01967
利用RIL群体和自然群体检测与花生含油量相关的SSR标记
Identification of SSR Markers Linked to Oil Content in Peanut (Arachis hypogaea L.) through RIL Population and Natural Population
摘要
Abstract
Oil content has been an important quality trait for peanut. However, the progress in generic improvement of oil content is slow. This is mainly due to the complex genetic basis, the high cost in oil content testing, and difficulty in phenotypic selection because of environmental influence. Therefore, marker assisted selection (MAS) is necessary for oil content improvement in peanut breeding. In the present study, F8 RILs derived from a cross of Yuanza 9102xZhonghua 5 were selected as materials. Ten RILs with extremely low oil content (about 52.91% on an average), twelve RILs with extremely high oil content (about 58.85% on an average) and the two parental lines were used to screen 631 simple sequence repeat (SSR) primers. Thirteen polymorphic bands of seven SSR primers were able to distinguish the extremely high and low oil content lines. Subsequently, the seven SSR primers were used to genotype the total RIL population. Based on the statistics of the SSR data from 20 lines with low oil content (less than 55%) and 45 lines with high oil content (more than 56%), we identified that a SSR marker, 2A5-250/240, was tightly linked to the oil content trait. The band 2A5-250 appeared almost in all low oil content lines with the match rate of 95.0%, while the band 2A5-240 appeared almost in all high oil content lines with the match rate of 88.9%. Detection of the marker 2A5-250/240 was conducted in 11 high oil (55.93% on an average) and 11 low oil (48.41% on an average) content peanut culti-vars. The results showed that the match rates of the band 2A5-250 to low oil content materials and 2A5-240 to high oil content materials was 90.9% and 63.6%, respectively. In addition, the band 2A5-240 appeared in 10 of the 19 wild peanut (Arachis) lines with high oil content (about 58.60%). The results based on the analyses using RIL and natural populations demonstrated that the SSR marker 2A5-250/240 was useful for marker-assisted selection of oil content in peanut.关键词
花生/含油量/SSR/分子辅助选择Key words
Peanut (Arachis hypogaea L.)/ Oil content/ SSR/ Marker assisted selection引用本文复制引用
黄莉,赵新燕,张文华,樊志明,任小平,廖伯寿,姜慧芳,陈玉宁..利用RIL群体和自然群体检测与花生含油量相关的SSR标记[J].作物学报,2011,37(11):1967-1974,8.基金项目
本研究由国家重点基础研究发展计划(973计划)(2011CB109300),农业部农作物种质资源保护项目(NB2010-2130135-28B),国家花生产业技术体系建设(CARS-14-种质资源评价)资助. （973计划）
