作物学报2011，Vol.37Issue(11)：1991-2000,10.DOI:10.3724/SP.J.1006.2011.01991
转苦瓜几丁质酶基因McCHIT1水稻及其稻瘟病抗性
Transgenic Rice Lines Harboring McCHIT1 Gene from Balsam Pear (Momordica charantia L.) and Their Blast Resistance
摘要
Abstract
Rice blast is one of the most important diseases damaging rice. It is important to explore a new way of improving blast resistance with introducing exogenous resistance genes in modern breeding. In the study, identification and screening of blast resistance in the transgenic rice offspring were conducted using methods of both seedling nursery artificial inoculation and natural infection in field nursery under the conditions of higher resistance selection pressure increased gradually from T2 generation. Seven stable and outstanding McCMT1-transforming rice lines with the most significant blast resistance were obtained in T5 gen-eration. Detected with 115 Magnaporthe grisea effective isolates from 26 physiological races belonging to seven groups, the re-sistance frequencies for the seven lines were 52.2-61.7%, with 15 percentage higher than those (36.5%) for the control Jinhui 35. Compared with the control, these lines increased the resistance to ZE race group, and improved the resistance to the races of group ZG and ZF as well as dominant group ZB. The McCHITI-transforming rice lines C36-2-1, C21-6-2, and C21-3-1 were the better combination of higher yield and resistance with seed setting rate of over 80%. The McCHITI gene is a certain broad-spectrum resistance gene. The disease-resistant lines with both broad-spectrum resistance and better yield traits can be obtained effectively by introducing McCHITI gene under gradually increased selection pressure and screening excellent resistance from resistant lines.关键词
转基因水稻/McCHIT1/稻瘟病/抗性Key words
Transgenic rice plants/ Balsam pear class I chitinase gene (McCHITI)/ Rice blast/ Resistance引用本文复制引用
张长伟,凌英华,桑贤春,李平,赵芳明,杨正林,李云峰,方立魁,何光华..转苦瓜几丁质酶基因McCHIT1水稻及其稻瘟病抗性[J].作物学报,2011,37(11):1991-2000,10.基金项目
本研究由国家转基因生物新品种培育科技重大专项(2008ZX08001-002)和重庆市重大攻关项目(CSTC,2010AA1019 （2008ZX08001-002）
CSTC,2010AA1013)资助. （）
